Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA250385

Local Sample ID:	1-5
Subject ID:	SU002596
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Treatment:

Treatment ID:	TR002608
Treatment Summary:	Phenylhydrazine (PHZ)-induced hemolytic anemia To induce acute hemolytic anemia, age- and sex-matched mice were injected intraperitoneally with a single dose (100 mg/kg body weight) of freshly prepared phenylhydrazine (Sigma-Aldrich, dissolved in PBS). Mice were sacrificed at indicated time points for spleen sample collection and Kit cell enrichment. Brucella abortus-induced inflammatory anemia Heat-killed Brucella abortus (HKBA, strain 1119-3) was used to induce anemia of inflammation following a previously described method(1). To induce stress erythropoiesis, age- and sex-matched mice were administered with 5 x 108 particles of HKBA via intraperitoneal injection.Mice were sacrificed at indicated time points for spleen sample collection and Kit cell enrichment.

Treatment ID:

TR002608

Treatment Summary:

Phenylhydrazine (PHZ)-induced hemolytic anemia To induce acute hemolytic anemia, age- and sex-matched mice were injected intraperitoneally with a single dose (100 mg/kg body weight) of freshly prepared phenylhydrazine (Sigma-Aldrich, dissolved in PBS). Mice were sacrificed at indicated time points for spleen sample collection and Kit cell enrichment. Brucella abortus-induced inflammatory anemia Heat-killed Brucella abortus (HKBA, strain 1119-3) was used to induce anemia of inflammation following a previously described method(1). To induce stress erythropoiesis, age- and sex-matched mice were administered with 5 x 108 particles of HKBA via intraperitoneal injection.Mice were sacrificed at indicated time points for spleen sample collection and Kit cell enrichment.