Summary of Study ST001065

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000713. The data can be accessed directly via it's Project DOI: 10.21228/M8HM48 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001065
Study TitleLipidomics analysis for aged mice brain cortex (part-II)
Study TypeMS lipidomics analysis
Study SummaryLipidomics studies on samples from aged and young mice.
Takeda Pharmaceutical Company Limited
Last NameAndo
First NameAyumi
Address26-1, Muraoka-Higashi 2-chome, Fujisawa-shi, Kanagawa 251-8555, Japan
Submit Date2018-09-30
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2019-10-11
Release Version1
Ayumi Ando Ayumi Ando application/zip

Select appropriate tab below to view additional metadata details:


Project ID:PR000713
Project DOI:doi: 10.21228/M8HM48
Project Title:Lipidomics analysis for aged mice organs
Project Type:MS lipidomics analysis
Project Summary:Lipidomics analysis for aged mice organs
Institute:Takeda Pharmaceutical Company Limited
Last Name:Ando
First Name:Ayumi
Address:26-1, Muraoka-Higashi 2-chome, Fujisawa-shi, Kanagawa, 251-8555, Japan


Subject ID:SU001109
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J mice
Age Or Age Range:8 week (“young”) and 112 week (“old”).
Weight Or Weight Range:young: 20.2g (mean), aged: 34.3g (mean)
Animal Animal Supplier:CLEA (Tokyo, Japan)
Animal Light Cycle:a 12-hour light/12-hour dark schedule
Animal Feed:CE-2, CLEA
Animal Water:tap water


Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Age Tissue
SA071942Cortex_60_agedAged 112 weeks Cerebral Cortex
SA071943Cortex_58_agedAged 112 weeks Cerebral Cortex
SA071944Cortex_56_agedAged 112 weeks Cerebral Cortex
SA071945Cortex_54_agedAged 112 weeks Cerebral Cortex
SA071946Cortex_52_agedAged 112 weeks Cerebral Cortex
SA071947Adipose_60_agedAged 112 weeks Epididymal adipose tissue
SA071948Adipose_58_agedAged 112 weeks Epididymal adipose tissue
SA071949Adipose_52_agedAged 112 weeks Epididymal adipose tissue
SA071950Adipose_54_agedAged 112 weeks Epididymal adipose tissue
SA071951Adipose_56_agedAged 112 weeks Epididymal adipose tissue
SA071952Femoral_52_agedAged 112 weeks Femoral muscle
SA071953Femoral_58_agedAged 112 weeks Femoral muscle
SA071954Femoral_60_agedAged 112 weeks Femoral muscle
SA071955Femoral_56_agedAged 112 weeks Femoral muscle
SA071956Femoral_54_agedAged 112 weeks Femoral muscle
SA071957Liver_60_agedAged 112 weeks Liver
SA071958Liver_58_agedAged 112 weeks Liver
SA071959Liver_56_agedAged 112 weeks Liver
SA071960Liver_52_agedAged 112 weeks Liver
SA071961Liver_54_agedAged 112 weeks Liver
SA071962Cortex_59_youngYoung 8 weeks Cerebral Cortex
SA071963Cortex_57_youngYoung 8 weeks Cerebral Cortex
SA071964Cortex_51_youngYoung 8 weeks Cerebral Cortex
SA071965Cortex_55_youngYoung 8 weeks Cerebral Cortex
SA071966Cortex_53_youngYoung 8 weeks Cerebral Cortex
SA071967Adipose_59_youngYoung 8 weeks Epididymal adipose tissue
SA071968Adipose_57_youngYoung 8 weeks Epididymal adipose tissue
SA071969Adipose_55_youngYoung 8 weeks Epididymal adipose tissue
SA071970Adipose_51_youngYoung 8 weeks Epididymal adipose tissue
SA071971Adipose_53_youngYoung 8 weeks Epididymal adipose tissue
SA071972Femoral_57_youngYoung 8 weeks Femoral muscle
SA071973Femoral_59_youngYoung 8 weeks Femoral muscle
SA071974Femoral_55_youngYoung 8 weeks Femoral muscle
SA071975Femoral_53_youngYoung 8 weeks Femoral muscle
SA071976Femoral_51_youngYoung 8 weeks Femoral muscle
SA071977Liver_59_youngYoung 8 weeks Liver
SA071978Liver_57_youngYoung 8 weeks Liver
SA071979Liver_55_youngYoung 8 weeks Liver
SA071980Liver_53_youngYoung 8 weeks Liver
SA071981Liver_51_youngYoung 8 weeks Liver
Showing results 1 to 40 of 40


Collection ID:CO001103
Collection Summary:All mice were killed by decapitation for sample collection. Collected tissues were immediately frozen by liquid nitrogen then stored -80 C until use. (Femoral, Cortex, Liver, Adipose) tissues.
Sample Type:Brain
Storage Conditions:-80℃


Treatment ID:TR001123
Treatment Summary:Tissues were homogenised in isopropanol (100 mg/mL) for lipidomics.

Sample Preparation:

Sampleprep ID:SP001116
Sampleprep Summary:Tissues were homogenised in isopropanol (100 mg/mL) for lipidomics.

Combined analysis:

Analysis ID AN001742 AN001743
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Orbitrap XL Orbitrap XL
Column Waters XBridge C18 (50 x 2.1mm,3um) Waters XBridge C18 (50 x 2.1mm,3um)
MS instrument type Single quadrupole Single quadrupole
MS instrument name Thermo LTQ XL Thermo LTQ XL
Units peak area peak area


Chromatography ID:CH001231
Chromatography Summary:The liquid chromatography/mass spectrometry system consisted of an Ultimate3000 RSLC system (Thermo Fisher Scientific inc., Sunnyvale, CA, USA) using a gradient elution consisting of mobile phase A (0.01% acetic acid and 1 mM ammonia in MilliQ water) and mobile phase B (0.001% acetic acid and 0.2 mM ammonia in ethanol/isopropanol [3:1])
Instrument Name:Orbitrap XL
Column Name:Waters XBridge C18 (50 x 2.1mm,3um)
Solvent A:100% water; 0.01% acetic acid; 1 mM ammonia
Solvent B:75% ethanol/25% isopropanol; 0.001% acetic acid; 0.2 mM ammonia
Chromatography Type:Reversed phase


MS ID:MS001610
Analysis ID:AN001742
Instrument Name:Thermo LTQ XL
Instrument Type:Single quadrupole
MS ID:MS001611
Analysis ID:AN001743
Instrument Name:Thermo LTQ XL
Instrument Type:Single quadrupole