Summary of Study ST001190

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000802. The data can be accessed directly via it's Project DOI: 10.21228/M8139N This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001190
Study TitleSepsis-related metabolic changes in ileum, jejunum, skeletal muscle, liver and lung
Study SummaryRationale: Sepsis is a multi-organ disease affecting the ileum and jejunum (small intestine),liver, "skeletal muscle, and lung clinically. Recently, specific alterations in circulating metabolites have been found in patients with sepsis which are thought to contribute to the pathogenesis of disease. The specific metabolic "changes in the ileum, jejunum, liver, skeletal muscle, and lung have not previously been investigated." Methods: Live Pseudomonas aeruginosa isolated from a patient was given via IV catheter to pigs " to induce severe sepsis. Eighteen hours later, ileum, jejunum, medial gastrocnemius skeletal muscle, liver, " and lung were harvested and flash frozen. Tissues were subsequently processed for non-targeted metabolomics analysis using gas chromatography/mass spectrometry. "Results: After 18 hours of sepsis, the ileum and the liver demonstrated significant changes in " "metabolites involved in linoleic acid metabolism, the ileum and lung had significant changes in " "valine/leucine/isoleucine metabolism, the jejunum, skeletal muscle, and liver had significant changes in " "arginine/ proline metabolism, and the skeletal muscle and lung had significant changes in aminoacyl-tRNA " biosynthesis by pathway analysis. Pathway analysis also identified changes in metabolic pathways unique for "different tissues, including changes in the citric acid cycle (jejunum), beta-alanine metabolism (skeletal muscle), and purine metabolism (liver)." Conclusion: These findings demonstrate both overlapping metabolic pathways affected in different tissues and those that are unique to others and provide insight into the metabolic changes in sepsis leading to organ dysfunction. This may allow therapeutic interventions that focus on multiple tissues or single tissues once the relationship of the altered metabolites/metabolism to the underlying pathogenesis of sepsis is determined.
Institute
Indiana University School of Medicine
DepartmentIndiana Center for Musculoskeletal Health / Dept. of Pathology & Laboratory Medicine
LaboratoryMultiple Centers
Last NameWillis
First NameMonte
AddressNA
Emailmonte_willis@outlook.com
Phone(984) 999-5431
Submit Date2018-12-03
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2019-06-08
Release Version1
Monte Willis Monte Willis
https://dx.doi.org/10.21228/M8139N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001982
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890 N GC
Column Agilent DB-5ms (15m x 0.25 mm x 0.25 ┬Ám)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Ion Mode POSITIVE
Units Peak values (Log transformed)
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