Summary of Study ST002827

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001769. The data can be accessed directly via it's Project DOI: 10.21228/M82130 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002827
Study Title	Multi-assay nutritional metabolomics profiling of low vitamin A status versus adequacy is characterized by reduced plasma lipid mediators among lactating women in the Philippines: A pilot study.
Study Type	Case-control
Study Summary	Low vitamin A (VA) status is common among lactating women in low-income countries. Lactation has substantial effects on mother’s metabolism and VA is required in multiple biological processes, including growth, vision, immunity, and reproduction. The objective of this pilot study was to utilize metabolomics profiling to conduct a broad, exploratory assessment of differences in plasma metabolites associated with low VA status versus VA adequacy in lactating women. Plasma samples from lactating women who participated in a survey in Samar, Philippines, were selected from a cross-sectional study based on plasma retinol concentrations indicating low (VA-; n=5) or adequate (VA+; n=5) VA status (plasma retinol <0.8 or >1.05 µmol/L). The plasma results collected from six metabolomics assays (oxylipins, endocannabinoids, bile acids, primary metabolomics, biogenic amines, and lipidomics) were compared by group using liquid chromatography mass spectrometry. Twenty-eight metabolites were altered in the VA- versus VA+ status groups, with 24 being lipid mediators (p<0.05). These lipid mediators included lower concentrations of arachidonic acid- and eicosapentaenoic acid-derived oxylipins, as well as lysophospholipids and sphingolipids, in the VA- group (p<0.05). Chemical similarity enrichment analysis identified HETEs, HEPEs, and DiHETEs as significantly altered oxylipin clusters (p<0.0001, false discovery rate (FDR) p<0.0001), as well as sphingomyelins, saturated lysophosphatidylcholines, phosphatidylcholines, and phosphatidylethanolamines (p<0.001, FDR p<0.01). The multi-assay nutritional metabolomics profiling of low VA status compared with adequacy in lactating women was characterized by reduced lipid mediator concentrations. Future studies with stronger study designs and larger sample size are needed to confirm and validate these preliminary results.
Institute	California Polytechnic State University, San Luis Obispo
Department	Food Science and Nutrition
Laboratory	Cal Poly Metabolomics Service Center
Last Name	La Frano
First Name	Michael
Address	Attn: Dr. Michael La Frano Bldg 11 Room 239 Cal Poly State University 1 Grand Avenue San Luis Obispo, CA 93407
Email	mlafrano@calpoly.edu
Phone	(805) 756 6233
Submit Date	2023-03-24
Num Groups	2
Total Subjects	10
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	API
Release Date	2023-09-14
Release Version	1

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Combined analysis:

Analysis ID	AN004614	AN004615	AN004616
Analysis type	MS	MS	MS
Chromatography type	HILIC	HILIC	Reversed phase
Chromatography system	Waters Acquity I-Class	Waters Acquity I-Class	Waters Acquity I-Class
Column	Phenomenex Luna NH2 (150 x 2mm,3um)	Waters Atlantis HILIC (150 x 2.1 mm, 3um)	Prosphere HP C4 (150 x 3.0 mm, 3um)
MS Type	API	API	API
MS instrument type	QTRAP	QTRAP	QTRAP
MS instrument name	ABI Sciex API 4000 QTrap	ABI Sciex API 4000 QTrap	ABI Sciex API 4000 QTrap
Ion Mode	NEGATIVE	POSITIVE	POSITIVE
Units	Peak Area	Peak Area	Peak Area