Summary of Study ST000352
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000281. The data can be accessed directly via it's Project DOI: 10.21228/M8KW38 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000352 |
| Study Title | Metabolic profiling reveals biochemical pathways and potential biomarkers associated with the pathogenesis of Krabbe disease |
| Study Type | Animal hindbrain comparison |
| Study Summary | P15 wildtype mouse hindbrains, P15 twitcher mouse hindbrains, P22 wildtype mouse hindbrains, P22 twitcher mouse hindbrains |
| Institute | University at Buffalo |
| Department | Hunter James Kelly Research Institute |
| Laboratory | Shin Laboratory |
| Last Name | Shin |
| First Name | Daesung |
| Address | NYS Center of Excellence in Bioinformatics & Life Sciences, 701 Ellicott Street, Buffalo, NY 14203 |
| daesungs@buffalo.edu | |
| Phone | 716-881-8980 |
| Submit Date | 2016-02-02 |
| Num Groups | 4 |
| Total Subjects | 32 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2016-03-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000572 | AN000573 | AN000574 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Unspecified | Unspecified | GC |
| Chromatography system | Waters ACQUITY UPLC | Waters ACQUITY UPLC | See document |
| Column | See doc | See doc | See document |
| MS Type | ESI | ESI | EI |
| MS instrument type | Single quadrupole | Single quadrupole | Single quadrupole |
| MS instrument name | Thermo LTQ | Thermo LTQ | Thermo Trace DSQ |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | Peak height | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000409 |
| Chromatography Summary: | Experiments were conducted according to the protocols approved by the Institutional Animal Care and Use Committee of University at Buffalo and Roswell Park Cancer Institute. Twitcher mice were maintained on the background of C57BL/6N. Breeder C57BL/6N mice were purchased from Charles River. Freshly collected hindbrain regions from twitcher and WT littermate mice at P8, P15, P22, and P34 days were transferred into polypropylene cryovials, and then frozen in liquid nitrogen and stored at - 80°C until the analysis. |
| Methods Filename: | pathogenesis_Krabbe_protocol.pdf |
| Instrument Name: | Waters ACQUITY UPLC |
| Column Name: | See doc |
| Chromatography Type: | Unspecified |
| Chromatography ID: | CH000410 |
| Chromatography Summary: | Experiments were conducted according to the protocols approved by the Institutional Animal Care and Use Committee of University at Buffalo and Roswell Park Cancer Institute. Twitcher mice were maintained on the background of C57BL/6N. Breeder C57BL/6N mice were purchased from Charles River. Freshly collected hindbrain regions from twitcher and WT littermate mice at P8, P15, P22, and P34 days were transferred into polypropylene cryovials, and then frozen in liquid nitrogen and stored at - 80°C until the analysis. |
| Methods Filename: | pathogenesis_Krabbe_protocol.pdf |
| Instrument Name: | See document |
| Column Name: | See document |
| Solvent A: | 5% phenyl |
| Chromatography Type: | GC |
