Summary of Study ST000443
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000330. The data can be accessed directly via it's Project DOI: 10.21228/M8W02Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000443 |
| Study Title | Quantitative measurements of TCA cycle metabolites in T1D poor control, good control, and controls. |
| Study Type | Quantitative measurements of plasma levels of tricarboxylic acid (TCA) cycle metabolites |
| Study Summary | The objective of the study was to determine whether T1D with good glycemic control have persistent abnormalities of metabolites and pathways that exist in T1D with poor glycemic control. |
| Institute | Mayo Clinic |
| Department | Endocrinology |
| Laboratory | Mayo Clinic Metabolomics Resource Core |
| Last Name | Nair |
| First Name | Sreekumaran |
| Address | 200 First Street SW, Rochester, MN 55905 |
| Nair.K@mayo.edu | |
| Phone | 507-285-2415 |
| Submit Date | 2016-07-20 |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS |
| Release Date | 2016-09-23 |
| Release Version | 1 |
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Combined analysis:
| Analysis ID | AN000694 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890 |
| Column | None |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5973 |
| Ion Mode | POSITIVE |
| Units | micromolar |
Chromatography:
| Chromatography ID: | CH000502 |
| Chromatography Summary: | The derivatized extracts were analyzed with an Agilent 6890 gas chromatograph coupled with an Agilent 5973 mass selective detector. The 1-μL aliquots of the extracts were injected into a DB5-MS capillary column (30 m × 250 μm i.d., 0.25-μm film thickness; J&W Scientific, Folson, CA) using PTV injection (Gerstel CIS4 injector) in the splitless mode. The temperature of the PTV was 70 °C during injection, and 0.6 min after injection, the temperature was raised to 300 °C at a rate of 2 °C/s and held at 300 °C for 20 min. The initial GC oven temperature was 70 °C, 5 min after injection the GC oven temperature was increased with 5 °C/min to 320 °C and held for 5 min at 320 °C. Helium was used as a carrier gas and pressure programmed such that the helium flow was kept constant at a flow rate of 1.7 mL/min. Detection was achieved using MS detection in electron impact mode and full scan monitoring mode (m/z 15−800). The temperature of the ion source was set at 250 °C and that of the quadrupole at 200 °C. |
| Instrument Name: | Agilent 6890 |
| Column Name: | None |
| Chromatography Type: | GC |
