Summary of Study ST000834

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000595. The data can be accessed directly via it's Project DOI: 10.21228/M8RQ4G This work is supported by NIH grant, U2C- DK119886.


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Study IDST000834
Study TitlePurine and TCA measurements in salt sensitive (SS) hypertension
Study TypeMS analysis
Study SummarySS rats were surgically implanted with a chronic servo-control cuff, the purpose of which is to maintain normal pressure to the left kidney while the right kidney is exposed to high blood pressure. After 7 days of high salt treatment, which induces high blood pressure in the SS rat, rats were anesthetized with pentobarbital, kidneys were flushed and removed. The renal medulla was separated from the cortex using scissors, and the renal medullas were frozen in liquid nitrogen and stored at -80 C.
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups11
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS/LC-MS
Release Date2018-02-07
Release Version1
Maureen Kachman Maureen Kachman application/zip

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Combined analysis:

Analysis ID AN001424 AN001425
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent Agilent 7890N
Column Phenomenex Luna NH2 (150 x 1mm,3um) Agilent HP5-MS (15m × 0.25mm, 0.25 um)
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6520B QTOF Agilent 5975
Units counts/mg nmol/mg


Chromatography ID:CH000995
Chromatography Summary:Amino, Aminopropyl HILIC
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:HILIC
Chromatography ID:CH000996
Chromatography Summary:5% Phenyl Methyl Siloxane
Instrument Name:Agilent 7890N
Column Name:Agilent HP5-MS (15m × 0.25mm, 0.25 um)
Chromatography Type:GC