Summary of Study ST000966
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000663. The data can be accessed directly via it's Project DOI: 10.21228/M8ZX0W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000966 |
| Study Title | Metabolite and gene expression profiles suggest a putative mechanism through which high dietary carbohydrates reduce the content of hepatic betaine in Megalobrama amblycephala |
| Study Summary | plasma of Megalobrama amblycephala fed with control diet, high carbohydrates diet,betaine diet with 16 weeks and betaine diet with 4 weeks. |
| Institute | College of Fisheries Huazhong Agricultural University |
| Last Name | Xu |
| First Name | Jia |
| Address | 1 Shizishan Road Hongshan District Wuhan |
| xujia2018hzau@163.com | |
| Phone | 13018097215 |
| Submit Date | 2018-05-04 |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-06-05 |
| Release Version | 1 |
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Combined analysis:
| Analysis ID | AN001580 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Unspecified |
| Column | Unspecified |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | |
| Ion Mode | POSITIVE |
| Units | ng/ml or umol/L |
Chromatography:
| Chromatography ID: | CH001109 |
| Chromatography Summary: | The HPLC system consisted of a SRD-3600 Solvent Rack with analytical 6-channel vacuum degasser, a DGP-3600A pump, WPS-3000TSL analytical autosampler, and a tcc-3200 column compartment. Chromatographic separations were performed on an MSLab C18 column (150×4.6mm, 5μm). The mobile phase A was 0.1% formic acid in water, and the organic mobile phase B was 0.1% ammonium formate in acetonitrile with pH=5.8. The solvent was delivered to the column at a flow rate of 1 ml min−1 as follows: 0-1 minutes from A-B (90:10) to A-B (90:10); 1-12 minutes from A-B (90:10) to A-B (30:70); 12-12.1 minutes from A-B (30:70) to A-B (0:100); 12.1-15 minutes from A-B (0:100) to A-B (0:100); 15-15.1 minutes from A-B (0:100) to A-B (90:10); 15.1-20 minutes from A-B(90:10) to A-B (90:10). |
| Instrument Name: | Unspecified |
| Column Name: | Unspecified |
| Flow Gradient: | 0-1 minutes from A-B (90:10) to A-B (90:10); 1-12 minutes from A-B (90:10) to A-B (30:70); 12-12.1 minutes from A-B (30:70) to A-B (0:100); 12.1-15 minutes from A-B (0:100) to A-B (0:100); 15-15.1 minutes from A-B (0:100) to A-B (90:10); 15.1-20 minutes from A-B(90:10) to A-B (90:10). |
| Flow Rate: | 1ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% ammonium formate, pH 5.8 |
| Chromatography Type: | Reversed phase |
