Summary of Study ST001061
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000711. The data can be accessed directly via it's Project DOI: 10.21228/M8S383 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001061 |
| Study Title | Lipidomics of Near-Term Fetal and Newborn Sheep Cardiac Tissue (part-II) |
| Study Type | Comparison |
| Study Summary | Cardiac tissue from near-term fetal and newborn sheep were compared via NMR metabolomic analysis |
| Institute | University of Florida |
| Department | Biochemistry & Molecular Biology |
| Last Name | Walejko |
| First Name | Jacquelyn |
| Address | R3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
| jwalejko@ufl.edu | |
| Phone | na |
| Submit Date | 2018-09-10 |
| Num Groups | 2 |
| Total Subjects | 14 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-10-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001731 | AN001732 |
|---|---|---|
| Chromatography ID | CH001224 | CH001224 |
| MS ID | MS001600 | MS001601 |
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Relative peak area | Relative peak area |
Chromatography:
| Chromatography ID: | CH001224 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Column Temperature: | 4C |
| Flow Gradient: | 80% A and 20% B at 0 min; 80% A and 20% B at 1 min; 70% A and 30% B at 3 min; 55% A and 45% B at 4 min; 40% A and 60% B at 6 min; 35% A and 65% B at 8 min; 35% A and 65% B at 10 min; 10% A and 90% B at 15 min; 2% A and 98% B at 17 min; 2% A and 98% B at 18 min; 80% A and 20% B at 19 min; 80% A and 20% B at 23 min |
| Flow Rate: | 0.5mL/min |
| Sample Injection: | 2 uL |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/8% acetonitrile/2% water; 0.1% formic acid; 10 mM ammonium formate |
| Analytical Time: | 18 min |
| Chromatography Type: | Reversed phase |
