Summary of Study ST001093
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000731. The data can be accessed directly via it's Project DOI: 10.21228/M8638T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001093 |
| Study Title | Impact of genetic suppression (shRNA) of ALDH1A1 expression in human colon cancer cell line (COLO320) |
| Study Type | Untargeted metabolomics |
| Study Summary | Using a multi-omics approach, we have investigated the impact of genetic suppression (shRNA) of ALDH1A1 expression on transcriptomics, proteomics and untargeted metabolomics analyses in a human colon cancer cell line (COLO320). The present study (i) generates an integrative omic profile of scramble shRNA vs. ALDH1A1 shRNA COLO320 cells, and (ii) identifies possible alterations in biological pathways caused by suppression of ALDH1A1 expression. |
| Institute | Yale University |
| Last Name | Vasiliou |
| First Name | Vasilis |
| Address | 60 College str |
| georgia.charkoftaki@yale.edu; vasilis.vasiliou@yale.edu | |
| Phone | 203.737.8094 |
| Submit Date | 2018-11-12 |
| Num Groups | 2 |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-09-23 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001779 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters |
| Column | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Synapt G2 XS QTOF |
| Ion Mode | POSITIVE |
| Units | abundance corresponding to m/z |
Chromatography:
| Chromatography ID: | CH001257 |
| Chromatography Summary: | The mobile phase involved a gradient comprising varying amounts of solutions A (10 mM ammonium formate in 60% v/v acetonitrile) and B (10 mM ammonium formate in 95% isopropanol and 5% acetonitrile). The linear gradient elution was: 40–43% B (0–2 min), 43–50% B (over 0.1 min), 50–54% B (over 10 min), 54–70% B (over 0.1 min), 70–99% B (over 5.9 min), 99-40% B (over 0.1 min), and 40% B (for 2 min), run at 300 μl/min in positive Electrospray Ionization mode and the column temperature was maintained at 55 °C |
| Instrument Name: | Waters |
| Column Name: | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
| Flow Rate: | 300 μl/min |
| Injection Temperature: | 4 |
| Internal Standard: | SPLASH™ Lipidomix® Mass Spec Standard (Avanti Polar Lipids, Inc.) |
| Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate |
| Solvent B: | 95% isopropanol/5% acetonitrile; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
