Summary of Study ST001467

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000999. The data can be accessed directly via it's Project DOI: 10.21228/M8K10H This work is supported by NIH grant, U2C- DK119886.

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Study IDST001467
Study TitleMetabolites changes related to glucose-mediated energy production in chemotheraphy-induced cachexia
Study SummaryTargeted metabolomics platforms included amino acids and metabolites related to glucose-mediated energy production. The targeted metabolome changed with chemotheraphy-induced cachexia, and the changes were reversed with potential treatment of the cachexia. .rdb files were included as raw data files where detailed information regarding MRM transitions and internal standards can be found. Several amino acids (Gly, Pro, Gln, Taurine) were analyzed after dilution because their peak intensities were too high. Thus their analysis was performed separately from other amino acids, and their rdb files were saved in separate rdb files.
Institute
Asan Medical Center; University of Ulsan
Last NameYoo
First NameHyun Ju
Address88, Olympic-ro, 43-gil, Songpa-gu, Seoul 05505, South Korea
Emailyoohyunju@amc.seoul.kr
Phone82-02-3010-4029
Submit Date2020-08-18
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2020-09-21
Release Version1
Hyun Ju Yoo Hyun Ju Yoo
https://dx.doi.org/10.21228/M8K10H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002443
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290
Column Zorbax Eclipse XDB-C18 (100 x 2mm)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap
Ion Mode POSITIVE
Units area ratio

Chromatography:

Chromatography ID:CH001788
Chromatography Summary:Metabolites related to glucose-mediated metabolites were analyzed with LC-MS/MS (MRM)
Instrument Name:Agilent 1290
Column Name:Synergi fusion RP (50 x 2mm)
Column Temperature:23
Flow Gradient:0% B for 5 min, 0% to 90% B for 2 min, hold at 90% B for 8 min, 90% to 0% B for 1 min, and then hold at 0% B for 9 min
Flow Rate:70 μL/min except for minutes 7 to 15, when it was 140 µL/min
Internal Standard:13C5-glutamine
Sample Injection:3 uL
Solvent A:100% water; 5 mM ammonium acetate
Solvent B:100% acetonitrile; 5 mM ammonium acetate
Chromatography Type:Reversed phase
  
Chromatography ID:CH001789
Chromatography Summary:Amino acids and bioamines were analyzed with LC-MS/MS (MRM)
Instrument Name:Agilent 1290
Column Name:Zorbax Eclipse XDB-C18 (100 x 2mm)
Column Temperature:50
Flow Gradient:0% B for 0.5 min, 0% to 95% B for 5 min, hold at 95% B for 1 min, 95% to 0% B for 0.5 min, then hold at 0% B for 2.5 min
Flow Rate:500 μL/min
Internal Standard:13C5-glutamine, serotonine-d4, dopamine-d4, tryptophan-d5, serine-d3, and lysine-d8
Sample Injection:3 uL
Solvent A:100% water; 0.2% formic acid
Solvent B:100% acetonitrile; 0.2% formic acid
Chromatography Type:Reversed phase
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