Summary of Study ST001677
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001078. The data can be accessed directly via it's Project DOI: 10.21228/M8C111 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001677 |
| Study Title | Quantitative targeted measurements of sphingolipids in Th17 cells before and after the triple knockdown of SPTLC1,2,3 genes (SPT, de novo pathway: sphingolipid metabolism) (part-III) |
| Study Type | MS: Targeted analysis |
| Study Summary | Part 3/5: It includes quantitative targeted measurements of sphingolipids (ceramides and glycosphingolipids)in Th17 cells before(scrambled / control)and after the triple knockdown of SPTLC1,2,3 genes (SPT de novo pathway: sphingolipid metabolism). |
| Institute | University of Turku |
| Department | Systems Medicine, Turku Bioscience |
| Laboratory | Metabolomics |
| Last Name | Sen |
| First Name | Partho |
| Address | Tykistökatu 6B, BioCity, 5th Floor, Turku, Southwest, 20521, Finland |
| partho.sen@utu.fi | |
| Phone | 0469608145 |
| Submit Date | 2021-01-31 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-11-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002735 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity UHPLC UNSPSC 41115709 |
| Column | Waters BEH C18 (100 × 2.1 mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE |
| Units | ng/ml |
Chromatography:
| Chromatography ID: | CH002021 |
| Chromatography Summary: | Chromatographic separation was performed on an ACQUITY UHPLC BEH C18 column (2.1 mm × 100 mm, particle size 1.7 µm, Waters, Milford, MA, USA). The flow rate was set at 0.4 ml/min throughout the run with an injection volume of 1 µL. The following solvents were used for the gradient elution: Solvent A was H2O with 1% NH4Ac (1M) and HCOOH (0.1%) added. Solvent B was a mixture of ACN: IPA (1:1 v/v) with 1% NH4Ac (1M) and HCOOH (0.1%) added. The gradient was programmed as follows: 0 to 2 min 35-80% B, 2 to 7 min 80-100 % B, 7 to 14 min 100% B. The column was equilibrated with a 7min period of 35 % B prior to the next run. |
| Instrument Name: | Waters Acquity UHPLC UNSPSC 41115709 |
| Column Name: | Waters BEH C18 (100 × 2.1 mm,1.7um) |
| Flow Gradient: | The gradient was programmed as follows: 0 to 2 min 35-80% B, 2 to 7 min 80-100 % B, 7 to 14 min 100% B. The column was equilibrated with a 7min period of 35 % B prior to the next run. |
| Flow Rate: | 0.4ml/min |
| Solvent A: | 100% water; 0.1% formic acid; 1% ammonium acetate |
| Solvent B: | 50% acetonitrile/50% isopropanol; 0.1% formic acid; 1% ammonium acetate |
| Chromatography Type: | Reversed phase |
