Summary of Study ST001766

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001127. The data can be accessed directly via it's Project DOI: 10.21228/M81D6M This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001766
Study TitleApplication of the redox metabolite detection method for mammalian tissues (part I)
Study SummaryThis study was aimed at optimizing redox metabolites detection from mammalian tissues. Three different chromatographic conditions were compared as well as three different extraction conditions. This study was run on LUNA NH2 HILIC chromatography
Boston Children's Hospital, Harvard Medical School
LaboratoryNaama Kanarek
Last NamePetrova
First NameBoryana
Address300 Longwood Av, Boston, MA, 2115, USA
Submit Date2021-04-22
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-05-17
Release Version1
Boryana Petrova Boryana Petrova application/zip

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Combined analysis:

Analysis ID AN002873
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column Phenomenex Luna NH2 (150 x 2mm,3um)
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Units ppm


Chromatography ID:CH002128
Chromatography Summary:LUNA-NH2 chromatography: One  ml of reconstituted sample was injected into a Luna® 3 µm NH2 100 Å, LC Column (150x2 mm, 3 µm particle size; Phenomenex, 00F-4377-B0) operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific, San Jose, CA). Chromatographic separation was achieved using the following conditions: buffer A was acetonitrile; buffer B was 5 mM ammonium acetate and 0.2% ammonium hydroxide. Gradient conditions were: 20 min linear gradient from 10% to 90% B; 20-25 min hold at 90% B; 25–26 min from 90% to 10% B; 26–34 min hold at 10% B. The column oven and autosampler tray were held at 30 °C and 4 °C, respectively
Instrument Name:Thermo Vanquish
Column Name:Phenomenex Luna NH2 (150 x 2mm,3um)
Column Temperature:30
Flow Gradient:0 min linear gradient from 10% to 90% B; 20-25 min hold at 90% B; 25-26 min from 90% to 10% B; 26-34 min hold at 10% B.
Solvent A:100% acetonitrile
Solvent B:100% water; 5 mM ammonium acetate; 0.2% ammonium hydroxide
Chromatography Type:HILIC