Summary of Study ST001766
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001127. The data can be accessed directly via it's Project DOI: 10.21228/M81D6M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001766 |
| Study Title | Application of the redox metabolite detection method for mammalian tissues (part I) |
| Study Summary | This study was aimed at optimizing redox metabolites detection from mammalian tissues. Three different chromatographic conditions were compared as well as three different extraction conditions. This study was run on LUNA NH2 HILIC chromatography |
| Institute | Boston Children's Hospital, Harvard Medical School |
| Department | Pathology |
| Laboratory | Naama Kanarek |
| Last Name | Petrova |
| First Name | Boryana |
| Address | 300 Longwood Av, Boston, MA, 2115, USA |
| boryana.petrova@childrens.harvard.edu | |
| Phone | 6173557433 |
| Submit Date | 2021-04-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-05-17 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002873 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Phenomenex Luna NH2 (150 x 2mm,3um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | ppm |
Chromatography:
| Chromatography ID: | CH002128 |
| Chromatography Summary: | LUNA-NH2 chromatography: One ml of reconstituted sample was injected into a Luna® 3 µm NH2 100 Å, LC Column (150x2 mm, 3 µm particle size; Phenomenex, 00F-4377-B0) operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific, San Jose, CA). Chromatographic separation was achieved using the following conditions: buffer A was acetonitrile; buffer B was 5 mM ammonium acetate and 0.2% ammonium hydroxide. Gradient conditions were: 20 min linear gradient from 10% to 90% B; 20-25 min hold at 90% B; 25–26 min from 90% to 10% B; 26–34 min hold at 10% B. The column oven and autosampler tray were held at 30 °C and 4 °C, respectively |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Luna NH2 (150 x 2mm,3um) |
| Column Temperature: | 30 |
| Flow Gradient: | 0 min linear gradient from 10% to 90% B; 20-25 min hold at 90% B; 25-26 min from 90% to 10% B; 26-34 min hold at 10% B. |
| Solvent A: | 100% acetonitrile |
| Solvent B: | 100% water; 5 mM ammonium acetate; 0.2% ammonium hydroxide |
| Chromatography Type: | HILIC |
