Summary of Study ST001836
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001157. The data can be accessed directly via it's Project DOI: 10.21228/M8510S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001836 |
| Study Title | Quantitative lipids study on blood serum and ceacal content of rat models |
| Study Type | blood serum and caecum content |
| Study Summary | Lipids were analyzed in blood serum and cecal content in rat models |
| Institute | Helmholtz Centre for Environmental Research |
| Department | Department of Molecular Systems biology |
| Laboratory | Functional Metabolomics |
| Last Name | Rolle-Kampczyk |
| First Name | Ulrike |
| Address | Permoserstrasse 15, 04318 Leipzig, Germany |
| ulrike.rolle-kampczyk@ufz.de | |
| Phone | 0049 341 235 1537 |
| Submit Date | 2021-06-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-07-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002978 |
|---|---|
| Chromatography ID | CH002207 |
| MS ID | MS002768 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | UPLC (Waters Acquity, Waters Corporation, Milford, USA) |
| Column | Agilent Zorbax Eclipse XDB C18 (100 x 3.0mm,3.5um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE |
| Units | µM |
Chromatography:
| Chromatography ID: | CH002207 |
| Chromatography Summary: | LC - Instrument Parameters AbsoluteIDQ® p180 Kit (Biocrates Life Science AG, Innsbruck, Austria) System: UPLC (Waters Acquity, Waters Corporation, Milford, USA) Column: Agilent, Zorbax Eclipse XDB C18, 3.0 x 100 mm, 3.5 μM, Agilent Waldbronn, Germany Precolumn: Security Guard, Phenomenex, C18, 4 x 3 mm; Phenomenex, Aschaffenburg, Germany Materials: Water MiliQ, Methanol (Merck KGaA, Darmstadt, Germany, hypergrade for LC-MS) Acetonitril (Merck KGaA, Darmstadt, Germany hypergrade for LC-MS) Ammonium Acetate (Honeywell - Fluka, Seelze, Germany) Formic Acid (Honeywell, Fluka, Seelze, Germany) Running Solvent: 5mM ammonium acetat in methanol LC: A: 2mL Formic acid in MilliQ water B: 1mL Formic Acid in Acetonitrile Gradient Table LC Time (min) Flow Rate (mL/min) %A %B Curve Initial 0.500 100.0 0.0 Initial 0.50 0.500 100.0 0.0 6 4.00 0.500 30.0 70.0 6 5.30 0.500 30.0 70.0 6 5.40 0.500 100.0 0.0 6 7.30 0.500 100.0 0.0 6 |
| Methods Filename: | LC_parameters-metabolites.pdf |
| Instrument Name: | UPLC (Waters Acquity, Waters Corporation, Milford, USA) |
| Column Name: | Agilent Zorbax Eclipse XDB C18 (100 x 3.0mm,3.5um) |
| Flow Gradient: | FIA Time (min) Flow Rate (mL/min) %A %B Curve Initial 0.030 0.0 100.0 Initial 1.60 0.030 0.0 100.0 6 2.40 0.200 0.0 100.0 6 2.80 0.200 0.0 100.0 6 3.00 0.030 0.0 100.0 6 |
| Flow Rate: | 0.03ml/min |
| Solvent A: | 100% methanol; 5mM ammonium acetate |
| Solvent B: | 50% methanol/50% water; 5mM ammonium acetate |
| Chromatography Type: | Reversed phase |
