Summary of Study ST002012

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001276. The data can be accessed directly via it's Project DOI: 10.21228/M8S70F This work is supported by NIH grant, U2C- DK119886.

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Study IDST002012
Study TitleUntargeted primary metabolite profiling in Arabidopsis thaliana
Study SummaryThe goal of this project is to compare the primary metabolite profile in different tissue types of the model plant Arabidopsis thaliana. Specifically, plants were grown hydroponically under the long-day (16hr light/day) condition at 21C. Tissue samples, including leaves, inflorescences, and roots were harvest 4 1/2 weeks post sowing. Untargeted primary metabolites profiling was carried out using GCTOF.
Institute
Salk Institute for Biological Studies
DepartmentPBIO
LaboratoryJoanne Chory
Last NameWu
First NameXuelin
AddressSalk Institute for Biological Studies
Emailxwu@salk.edu
Phone858-453-4100, x1128
Submit Date2021-12-09
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2022-01-02
Release Version1
Xuelin Wu Xuelin Wu
https://dx.doi.org/10.21228/M8S70F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003286
Analysis type MS
Chromatography type GC
Chromatography system Gerstel CIS4 –with dual MPS Injector/ Agilent 6890 GC- Pegasus III TOF MS
Column Rtx-5Sil MS
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus IV TOF
Ion Mode UNSPECIFIED
Units normalized peak height

Chromatography:

Chromatography ID:CH002426
Chromatography Summary:Gas Chromatography conditions: A 30 m long, 0.25 mm i.d. Rtx-5Sil MS column (0.25 μm 95% dimethyl 5% diphenyl polysiloxane film) with additional 10 m integrated guard column is used (Restek, Bellefonte PA). 99.9999% pure Helium with built-in purifier (Airgas, Radnor PA) is set at constant flow of 1 ml/min. The oven temperature is held constant at 50°C for 1 min and then ramped at 20°C/min to 330°C at which it is held constant for 5 min.
Instrument Name:Gerstel CIS4 –with dual MPS Injector/ Agilent 6890 GC- Pegasus III TOF MS
Column Name:Rtx-5Sil MS
Chromatography Type:GC
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