Summary of Study ST002269

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001451. The data can be accessed directly via it's Project DOI: 10.21228/M85H8N This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002269
Study TitleTargeted plasma metabolomics in pediatric NAFLD patients
Study SummarySeveral adult omics studies have been conducted to understand the pathophysiology of nonalcoholic fatty liver disease (NAFLD). However, the histological features of children are different from those of adults, and the onset and progression of pediatric NAFLD are not fully understood. In this study, we aimed to evaluate the metabolome profile and metabolic pathway changes associated with pediatric NAFLD to elucidate its pathophysiology. We analyzed the metabolic profiles of healthy control, lean NAFLD, overweight control and overweight NAFLD groups of children and adolescent participants (n = 165) by assessing plasma samples, and identified 18 NAFLD-specific metabolic features and metabolic changes in lipid, glutathione-related amino acid, and branched-chain amino acid metabolism by comparing control and NAFLD group in pediatric population with overweight. Metabolome changes in the plasma of pediatric patients with NAFLD are associated with the pathophysiology of the disease and can be utilized as a less-invasive approach to diagnose the disease.
Institute
Seoul National University College of Medicine and Hospital
DepartmentDepartment of Clinical Pharmacology and Therapeutics
Last NameChae
First NameWoori
Address101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea
Emailyunus@snu.ac.kr
Phone82-2-740-8905
Submit Date2022-08-07
Num Groups4
Total Subjects165
Num Males122
Num Females43
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS/MS(Dir. Inf.)
Release Date2023-08-07
Release Version1
Woori Chae Woori Chae
https://dx.doi.org/10.21228/M85H8N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003706 AN003707 AN003708 AN003709
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase None (Direct infusion) None (Direct infusion)
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032) Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032) NA NA
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE POSITIVE POSITIVE POSITIVE
Units μM μM μM μM

Chromatography:

Chromatography ID:CH002745
Chromatography Summary:LC1 method for analysis of amino acids and biogenic amines For more details, please refer sections 4.6, 4.7, 5, 9, 10.1, and 10.2 of the method file attached.
Methods Filename:UM-p400-HR-Thermo-3.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032)
Column Temperature:50
Solvent A:100% water; 0.2% formic acid
Solvent B:100% acetonitrile; 0.2% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH002746
Chromatography Summary:LC2 method for analysis of isoleucine For more details, please refer sections 4.6, 4.7, 5, 9, 10.1, and 10.2 of the method file attached.
Methods Filename:UM-p400-HR-Thermo-3.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032)
Column Temperature:50
Solvent A:100% water; 0.2% formic acid
Solvent B:100% acetonitrile; 0.2% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH002747
Chromatography Summary:FIA1 method for analysis of acylcarnitines and lipids For more details, please refer sections 4.6, 4.7, 5, 9, 10.1, and 10.2 of the method file attached.
Methods Filename:UM-p400-HR-Thermo-3.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:NA
Solvent A:290mL Methanol + 1 ampule FIA mobile phase additive
Chromatography Type:None (Direct infusion)
  
Chromatography ID:CH002748
Chromatography Summary:FIA2 method for analysis of acylcarnitines For more details, please refer sections 4.6, 4.7, 5, 9, 10.1, and 10.2 of the method file attached.
Methods Filename:UM-p400-HR-Thermo-3.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:NA
Solvent A:290mL Methanol + 1 ampule FIA mobile phase additive
Chromatography Type:None (Direct infusion)
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