Summary of Study ST002581
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002581 |
| Study Title | MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Plasma - Targeted Keto Acids |
| Study Summary | The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group. |
| Institute | Duke University |
| Department | Duke Molecular Physiology Institute |
| Laboratory | Metabolomics Core Laboratory |
| Last Name | Newgard |
| First Name | Christopher |
| Address | 300 N Duke St, Durham, NC 27701 |
| chris.newgard@duke.edu | |
| Phone | (919) 668-6059 |
| Submit Date | 2023-04-25 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | TBD |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-10-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004252 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity UPLC |
| Column | Waters Acquity UPLC BEH C18 Column, 1.7 µm, 2.1 × 50 mm |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Waters Xevo TQ-S |
| Ion Mode | POSITIVE |
| Units | micromole/L |
Chromatography:
| Chromatography ID: | CH003158 |
| Chromatography Summary: | The analytical column is used at 30°C. 10 µl of the sample are injected onto the column and eluted at a flow rate of 0.4 ml/min. The gradient begins with 45% eluent A (5 mM ammonium acetate in water) and is then programmed as follows: 0 to 2 min – gradient to 55% eluent B (methanol); 2 to 2.5 min - gradient to 95% eluent B; 2.5 to 3.2 min - hold at 95% eluent B, return to 45% A, and re-equilibrate the column at initial conditions for 1 minute. |
| Methods Filename: | pass1b_ka_methods.pdf |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | Waters Acquity UPLC BEH C18 Column, 1.7 µm, 2.1 × 50 mm |
| Column Temperature: | 30C |
| Flow Gradient: | 45% A and 55% B for 2 minutes, followed by a linear gradient to 95% B from 2 to 2.5 minutes, held at 95% B for 0.7 minutes, returned to 45% A, and finally the column was re-equilibrated at initial conditions for 1 minute. The total run time was 4.7 minutes. |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | 100% water; 5 mM ammonium acetate |
| Solvent B: | 100% methanol |
| Chromatography Type: | Reversed phase |
