Summary of Study ST002724
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001690. The data can be accessed directly via it's Project DOI: 10.21228/M8813V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002724 |
Study Title | Ceramides profile in HeLa overexpressing nSMase2 (SMPD3) |
Study Type | Lipidomics LC-MS/MS |
Study Summary | This study aim to determine which species of ceramides are modified by overexpression of the protein neutral sphingomyelinase 2 (nSMase2, gene SMPD3). HeLa cells were transfected with a plasmid containing V5-tagged nSMase2. Lipids were extracted after 24h after transfection and the sphingolipid profile was determined by LC-MS/MS. |
Institute | State University of New York |
Department | MEdicine |
Laboratory | Daniel Canals |
Last Name | Daniel |
First Name | Canals |
Address | 100 Nicolls Road |
daniel.canals@stonybrook.edu | |
Phone | 6312162903 |
Submit Date | 2023-05-30 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-06-21 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004415 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Spectra C8SR (150 × 3.0 mm, 3um) (#S-3C8SR-FJ) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo Quantiva |
Ion Mode | POSITIVE |
Units | pmols |
Chromatography:
Chromatography ID: | CH003314 |
Instrument Name: | Thermo Vanquish |
Column Name: | Spectra C8SR (150 × 3.0 mm, 3um) (#S-3C8SR-FJ) |
Column Temperature: | 37 |
Flow Gradient: | 0.5ml/min. 70%B. 5 min 90%B, 17 min 99%B, 26min 99%B, 26.5min 70% B. Stop at 35min. |
Flow Rate: | 0.5ml/min |
Solvent A: | 100% water; 0.2% formic acid ; 1mM ammonium formate |
Solvent B: | 100% methanol; 0.2% formic acid ; 1mM ammonium formate |
Chromatography Type: | Reversed phase |