Summary of Study ST002779
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001734. The data can be accessed directly via it's Project DOI: 10.21228/M8K72B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002779 |
| Study Title | Contribution of sugar transporters to spatial microbiota colonization along the longitudinal root axis of Arabidopsis |
| Study Summary | Longitudinal gut-axes of animals show spatial heterogeneity of microbiota related to physiological differentiation. Plant roots show functional heterogeneity in cellular architecture, transcriptome, metabolic states, and immunity. We hypothesized that axial differentiation impacts spatial colonization by rhizobiota along the root axis. We developed two growth systems, ArtSoil and CD-Rhizotrons, to dissect Arabidopsis thaliana roots into three segments. We identified distinct rhizobiota communities in the segments, supporting spatial microbiota differentiation along the axis. Root metabolite profiling revealed differential enrichment and specificity. Correlation analyses point to strong reliance of rhizobiota on carbohydrate supply from the host. Bioinformatic analyses and GUS histochemistry indicate sugar and/or microbe-induced accumulation of SWEET2, 4, and 12 sugar uniporters. Profiling of root-segments in sweet mutants showed impaired spatial rhizobiota arrangement. Correlation analysis revealed complex interconnected metabolite-rhizobiota networks. This work uncovers interdependency between root physiology and microbiota colonization and a contribution of SWEETs to shaping local adaptation of root microbiota. |
| Institute | Heinrich Heine University Düsseldorf |
| Last Name | Westhoff |
| First Name | Philipp |
| Address | Universitätsstr. 1 40225 Düsseldorf |
| philipp.westhoff@hhu.de | |
| Phone | +49 211-8110926 |
| Submit Date | 2023-06-26 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2024-02-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004525 | AN004526 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | GC | GC |
| Chromatography system | Agilent 7890B | Agilent 7890B |
| Column | Agilent HP5-MS (30m x 0.25mm, 0.25um) | Agilent HP5-MS (30m x 0.25mm, 0.25um) |
| MS Type | EI | EI |
| MS instrument type | QTOF | Single quadrupole |
| MS instrument name | Agilent 7200 QTOF | Agilent 5977B |
| Ion Mode | POSITIVE | POSITIVE |
| Units | Relative intensity | Relative intensity |
Chromatography:
| Chromatography ID: | CH003399 |
| Chromatography Summary: | Dried samples were derivatized according to (Gu et al. 2012 DOI:10.1039/c2ib00109h) and measured as described (Shim et al. 2019 DOI:10.3389/fpls.2019.01726) |
| Instrument Name: | Agilent 7890B |
| Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25um) |
| Column Temperature: | 350 |
| Flow Gradient: | N/A for GC |
| Flow Rate: | N/A for GC |
| Solvent A: | N/A for GC |
| Solvent B: | N/A for GC |
| Chromatography Type: | GC |
| Solvent C: | N/A for GC |
