Summary of Study ST002815

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001748. The data can be accessed directly via it's Project DOI: 10.21228/M8RQ7M This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002815
Study TitleInvestigation of metabolism in hypertrophic cardiomyopathy - HILIC
Study SummaryWe propose the use of targeted metabolomics to define the metabolic and molecular pathways altered in 2 mouse models (R92W-TnT and R403Q-MHC) of hypertrophic cardiomyopathy (HCM) that span the spectrum of human disease (heart failure and sudden death), with the goal of identifying treatment targets. Parallel targeted metabolomics studies will be performed in heart tissue and plasma at rest and following inotrope stimulation. Since energy compromise is expected to be most marked when the heart is subject to increased workload, as is the case during high-intensity exercise or inotropic stimulation, we propose metabolomics studies in heart tissue and plasma, at rest and following inotrope stimulation. We anticipate that the results of these studies will allow us to move forward with further investigations into specific metabolites of interest as biomarkers, to be tested in HCM patients in future studies.
University of California, San Francisco
DepartmentHypertrophic Cardiomyopathy Center of Excellence
LaboratoryUCSF Smith Cardiovascular Research Building
Last NameAbraham
First NameMaria Roselle
Address555 Mission Bay Boulevard South, San Francisco, CA 94158
Submit Date2023-07-27
Num Groups2 groups of 2 (i.e., mutant vs control of two mouse models of HCM (R402Q-MyHC, R92W-TnT)
Total Subjects32
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2023-09-06
Release Version1
Maria Roselle Abraham Maria Roselle Abraham application/zip

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Combined analysis:

Analysis ID AN004582
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1290
Column Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
MS instrument type Triple TOF
MS instrument name ABI Sciex 6600 TripleTOF
Units peak heights


Chromatography ID:CH003443
Chromatography Summary:Column: Waters Acquity UPLC BEH Amide Column, 1.7 μm, 2.1 mm x 150 mm); Pre-column: Waters Acquity UPLC BEH Amide VanGuard pre-column (1.7 μm , 5 mm × 2.1 mm;)
Instrument Name:Agilent 1290
Column Name:Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:40C
Flow Gradient:0 min 100% (B), 0-2 min 100% (B), 2-7 min 70% (B), 7.7-9 min 40% (B), 9.5-10.25 min 30% (B), 10.25- 12.75 min 100% (B), 16.75 min 100% (B)
Flow Rate:0.4 mL/min
Solvent A:Ultrapure water with 10 mM ammonium formiate + 0.125% formic acid, pH 3
Solvent B:95:5 v/v acetonitrile:ultrapure water w/ 10 mM ammonium formiate + 0.125% formic acid, pH 3
Chromatography Type:HILIC