Summary of Study ST002961
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001842. The data can be accessed directly via it's Project DOI: 10.21228/M8MH8P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002961 |
| Study Title | Analysis of Metabolites Secreted from Fibroblast Young Cells |
| Study Summary | In this experimental study, we aimed to uncover the factors in young cell secretions that trigger the reverse aging of mid-old cells, co-culturing them and observing a striking transformation, although we could not identify the specific factors responsible for this rejuvenation |
| Institute | Ajou University Medical Center |
| Last Name | Kim |
| First Name | Young Hwa |
| Address | 206, World cup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea |
| skyblue32@nate.com | |
| Phone | +82-10-5153-3636 |
| Submit Date | 2023-11-01 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-11-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004862 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity UHPLC |
| Column | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm, 1.8um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Q Exactive™ Hybrid Quadrupole-Orbitrap MS coupled with a 1290 Infinity UHPLC |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003670 |
| Chromatography Summary: | See protocol file, LC_MS_method_metabolite.pdf |
| Methods Filename: | LC_MS_method_metabolite.pdf |
| Instrument Name: | Agilent 1290 Infinity UHPLC |
| Column Name: | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm, 1.8um) |
| Column Temperature: | 320℃ |
| Flow Gradient: | 2.5% solvent B in 5 min, 2.5–12.5% solvent B in 29 min, 12.5–25% solvent B in 11 min, 25–37.5% solvent B in 11 min, 37.5-80% solvent B in 0.1 min, holding at 80% of solvent B in 13.9 min, 80–2.5% solvent B in 0.1 min, 2.5% solvent B for 19.9 min |
| Flow Rate: | 0.2 mL/min |
| Solvent A: | 0.1% formic acid in water |
| Solvent B: | 0.1% formic acid in 80% acetonitrile |
| Chromatography Type: | Reversed phase |
