Summary of Study ST002970

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001849. The data can be accessed directly via it's Project DOI: 10.21228/M8QB0V This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002970
Study TitleUntargeted metabolomics of fatty alcohol production at time 90 min of bioconversion
Study SummaryWe demonstrated applications of this system using three different engineered Escherichia coli requiring different cofactors (fatty alcohol, bioluminescence light, and alkane biosynthesis). The results showed that the XR/lactose system could enhance the production of each system by ~2-4-fold. Untargeted metabolomics and transcriptomics analyses revealed that XR only enhanced pathways related to specific cofactor utilization.
Institute
Vidyasirimedhi Institute of Science and Technology
Last NameSutthaphirom
First NameChalermroj
Address555, Moo 1, Payubnai, Wangchan, Rayong, 21210, Thailand
Emailchalermroj.s_s19@vistec.ac.th
Phone(+66)063-9639915
Submit Date2023-11-06
Num Groups2
Total Subjects8
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2023-11-20
Release Version1
Chalermroj Sutthaphirom Chalermroj Sutthaphirom
https://dx.doi.org/10.21228/M8QB0V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004879
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 6560
Column Agilent Poroshell 120 HILIC-Z (150 x 2.1 mm, 2.7 µm)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6560 Ion Mobility
Ion Mode NEGATIVE
Units abundance

Chromatography:

Chromatography ID:CH003681
Instrument Name:Agilent 6560
Column Name:Agilent Poroshell 120 HILIC-Z (150 x 2.1 mm, 2.7 µm)
Column Temperature:35˚C
Flow Gradient:0 min: 96%B, 24 min: 65%B
Flow Rate:0.3 mL/min
Solvent A:10 mM ammonium acetate pH 9.0
Solvent B:10 mM ammonium acetate in 85% acetonitrile/water pH 9.0
Chromatography Type:HILIC
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