Summary of Study ST002970
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001849. The data can be accessed directly via it's Project DOI: 10.21228/M8QB0V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002970 |
Study Title | Untargeted metabolomics of fatty alcohol production at time 90 min of bioconversion |
Study Summary | We demonstrated applications of this system using three different engineered Escherichia coli requiring different cofactors (fatty alcohol, bioluminescence light, and alkane biosynthesis). The results showed that the XR/lactose system could enhance the production of each system by ~2-4-fold. Untargeted metabolomics and transcriptomics analyses revealed that XR only enhanced pathways related to specific cofactor utilization. |
Institute | Vidyasirimedhi Institute of Science and Technology |
Last Name | Sutthaphirom |
First Name | Chalermroj |
Address | 555, Moo 1, Payubnai, Wangchan, Rayong, 21210, Thailand |
chalermroj.s_s19@vistec.ac.th | |
Phone | (+66)063-9639915 |
Submit Date | 2023-11-06 |
Num Groups | 2 |
Total Subjects | 8 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2023-11-20 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004879 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 6560 |
Column | Agilent Poroshell 120 HILIC-Z (150 x 2.1 mm, 2.7 µm) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6560 Ion Mobility |
Ion Mode | NEGATIVE |
Units | abundance |
Chromatography:
Chromatography ID: | CH003681 |
Instrument Name: | Agilent 6560 |
Column Name: | Agilent Poroshell 120 HILIC-Z (150 x 2.1 mm, 2.7 µm) |
Column Temperature: | 35˚C |
Flow Gradient: | 0 min: 96%B, 24 min: 65%B |
Flow Rate: | 0.3 mL/min |
Solvent A: | 10 mM ammonium acetate pH 9.0 |
Solvent B: | 10 mM ammonium acetate in 85% acetonitrile/water pH 9.0 |
Chromatography Type: | HILIC |