Summary of Study ST003041

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001283. The data can be accessed directly via it's Project DOI: 10.21228/M8VX1B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003041
Study TitleSoil pH, developmental stages and geographical origin differently influence the root metabolomic diversity and root-related microbial diversity of Echium vulgare from native habitats
Study SummaryThis study examines the impact of different factors (geographic location, soil pH, genetic cluster, developmental stage) on the metabolome and microbiome of wild Echium vulgare, as well as the microbiome-metabolome correlation contained therein. Samples analyzed are wild Echium vulgare plant roots collected at six locations in Austria; out of the six locations, four contain plant roots from two different developmental stages. Extraction of metabolites and subsequent UHPLC-HRMS analysis were performed to obtain data for metabolomics analysis.
Institute
Aristotle University of Thessaloniki
Last NameRodić
First NameNebojša
AddressStepe Stepanovića 5, Čonoplja, Vojvodina, Serbia
Emailnebojsa.rodic@hotmail.com
Phone+381648766400
Submit Date2024-01-05
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-01-16
Release Version1
Nebojša Rodić Nebojša Rodić
https://dx.doi.org/10.21228/M8VX1B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004988
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Accela AS
Column Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo LTQ Discovery Orbitrap
Ion Mode POSITIVE
Units Intensity

Chromatography:

Chromatography ID:CH003768
Instrument Name:Thermo Accela AS
Column Name:Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um)
Column Temperature:50
Flow Gradient:0 min 95A/5B, 1 min 50A/50B, 8 min 0A/100B, 13 min 0A/100B, 13.01 min 95A/5B, 16 min 95A/5B
Flow Rate:0.3 mL/min
Solvent A:Ultrapure water (0.1% formic acid)
Solvent B:LCMS-grade methanol (0.1% formic acid)
Chromatography Type:Reversed phase
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