Summary of Study ST000458
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000354. The data can be accessed directly via it's Project DOI: 10.21228/M8930Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000458 |
| Study Title | Effect of BCAT1 knockdown on amino acid steady state levels of Raji cells |
| Study Type | Cells are treated with vehicle or Doxycycline - to induce expression of BCAT1 short hairpin RNA. |
| Study Summary | Cells were collected at 0, 48, 96 and 144 hours after Doxycycline addition |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | John Cleveland |
| Last Name | Fernandez |
| First Name | Mario |
| Address | 12902 Magnolia Drive, MRC 4-West |
| mario.fernandez@moffitt.org | |
| Phone | 813-745-5140 |
| Submit Date | 2016-09-08 |
| Num Groups | 7 |
| Total Subjects | 21 |
| Study Comments | Groups collected in triplicate per time point, either treated or untreated with Doxycycline. |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-07-08 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO000473 |
| Collection Summary: | Cells were counted, 2e6 cells were collected via centrifugation, washed twice with cold 1x PBS, PBS removed and flash frozen. Pellets were saved at -80 C. |
| Sample Type: | Cell |
| Collection Method: | Pellet |
| Collection Location: | Lab |
| Collection Frequency: | Every 48 hours, up to 144 hours |
| Volumeoramount Collected: | 2e6 cells for 0, 48 and 144 time point. 5e6 cells for 96 hour time point |
| Storage Conditions: | Pellet |
| Storage Vials: | 1.5 mL microfuge tube |
| Tissue Cell Identification: | Raji cells |
