Summary of Study ST000468

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000360. The data can be accessed directly via it's Project DOI: 10.21228/M8HK59 This work is supported by NIH grant, U2C- DK119886.


Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files
Study IDST000468
Study TitleEnterococcus faecalis nucleotide profiles following mupirocin or decoyinine
Study TypeExposure to mupirocin or decoyinine
Study SummaryTriplicate samples of E. faecalis OG1RF will be grown in FMC-AUG to OD600 = 0.25. The cultures will be split and exposed to DMSO, mupirocin (0.01mg/mL) or decoyinine (0.1mg/mL) for 15 minutes. 6E9 cells will be harvested by fast filtration, scraped off of membrane into ice-cold PBS, and pelleted by centrifugation, and stored at -80C until shipment.
University of Florida
Last NameKajfasz
First NameJessica
Address1395 Center Drive, PO Box 100424 Gainesville, FL 32610
Submit Date2016-09-08
Num Groups3
Total Subjects9
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-10-03
Release Version1
Jessica Kajfasz Jessica Kajfasz application/zip

Select appropriate tab below to view additional metadata details:


Collection ID:CO000483
Collection Summary:After the 15 minute treatment interval, 20mL sample will be harvested by filtration onto 0.45uM nitrocellulose membrane. The cells will be scraped off of the membrane into ice-cold PBS (~1mL), centrifuged for 45 seconds at 12K RPM, liquid aspirated and stored at -80C until sending for analysis.
Sample Type:Bacterial cells
Collection Method:Fast filtration
Volumeoramount Collected:20ml
Storage Conditions:-80C