Summary of Study ST000468
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000360. The data can be accessed directly via it's Project DOI: 10.21228/M8HK59 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000468 |
Study Title | Enterococcus faecalis nucleotide profiles following mupirocin or decoyinine |
Study Type | Exposure to mupirocin or decoyinine |
Study Summary | Triplicate samples of E. faecalis OG1RF will be grown in FMC-AUG to OD600 = 0.25. The cultures will be split and exposed to DMSO, mupirocin (0.01mg/mL) or decoyinine (0.1mg/mL) for 15 minutes. 6E9 cells will be harvested by fast filtration, scraped off of membrane into ice-cold PBS, and pelleted by centrifugation, and stored at -80C until shipment. |
Institute | University of Florida |
Department | SECIM |
Laboratory | Lemos-Abranches |
Last Name | Kajfasz |
First Name | Jessica |
Address | 1395 Center Drive, PO Box 100424 Gainesville, FL 32610 |
jkajfasz@dental.ufl.edu | |
Phone | 352-273-6675 |
Submit Date | 2016-09-08 |
Num Groups | 3 |
Total Subjects | 9 |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-03 |
Release Version | 1 |
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Collection:
Collection ID: | CO000483 |
Collection Summary: | After the 15 minute treatment interval, 20mL sample will be harvested by filtration onto 0.45uM nitrocellulose membrane. The cells will be scraped off of the membrane into ice-cold PBS (~1mL), centrifuged for 45 seconds at 12K RPM, liquid aspirated and stored at -80C until sending for analysis. |
Sample Type: | Bacterial cells |
Collection Method: | Fast filtration |
Volumeoramount Collected: | 20ml |
Storage Conditions: | -80C |