Summary of Study ST000624
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000450. The data can be accessed directly via it's Project DOI: 10.21228/M8WC8S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000624 |
Study Title | Human trabecular meshowrk phospholipid profiles of control and glaucomatous eyes |
Study Summary | We compared phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and phosphatidylinositol) profiles of control and glaucomatous trabecular meshwork (TM) derived from human donors.Lipid extraction was performed using a modification of the Bligh and Dyer method, protein concentrations were determined using the Bradford method, and for select samples confirmed with densitometry of PHAST gels. Lipids were identified and subjected to ratiometric quantification using a TSQ quantum Access Max triple quadrupole mass spectrometer with precursor ion scan (PIS) or neutral ion loss scan (NLS), using appropriate class specific lipid standards. |
Institute | University of Miami |
Last Name | Bhattacharya |
First Name | Sanjoy |
Address | McKnight Vision Research Building, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 1638 NW 10th Avenue, Room 706A, Miami, Florida 33136 |
SBhattacharya@med.miami.edu | |
Phone | 305-482-4103 |
Submit Date | 2017-06-01 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | MS(Dir. Inf.) |
Release Date | 2019-07-17 |
Release Version | 1 |
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Collection:
Collection ID: | CO000641 |
Collection Summary: | Donor tissues were used following the institutional review board approved protocols and adhering to the tenets of the Declaration of Helsinki. Control TM and a subset of primary open-angle glaucoma (POAG) TM were collected from cadaver donors, a limited subset of POAG surgical specimen (acquired under institutional review board approved/exempted protocols) also were used for these studies. Cadaver donor tissues were sourced from Midwest Eye-Banks, Lions Eye Bank (Miami, FL), National Disease Research Interchange (NDRI; Philadelphia, PA), and Mundorf Eye Institute (Charlotte, NC). The postmortem time ranged from 3 to 16 hours. Control TM was stored briefly in Optisol at 48 and then used for lipid extraction. POAG TM tissues were stored at -80˚C until time of use. The mean age of control donors was 58.2 +/- 13.7 years and the mean age of glaucomatous TM donors 66 +/- 9.5 years. |
Sample Type: | Eye tissue |