Summary of Study ST000875
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000606. The data can be accessed directly via it's Project DOI: 10.21228/M82D92 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000875 |
| Study Title | Metabolomic investigations on Nesterenkonia flava from different origins revealed significant intraspecies differences between marine and terrestrial actinomycetes |
| Study Summary | Marine is one of the most important resources of microorganisms, including bacteria, actinomycetes, and fungi. As marine and terrestrial environments differ a lot in many aspects it is not surprising that the species and characteristics of microorganisms living there are very different. Interestingly, many marine microorganisms can find their congeners of the same species from terrestrial resources. The aim of this work is to evaluate the intraspecies differences between marine and terrestrial actinomycetes on metabolic level and to uncover the mechanism responsible for the differences. To address this, we carried out comparative metabolomics study on Nesterenkonia flava strains isolated from marine and terrestrial environments. The results showed that marine strains were clearly distinguished from their terrestrial congeners on the principal components analysis (PCA) scores plot of intracellular metabolites. The markers responsible for the discrimination of marine and terrestrial strains were figured out using loading plot from partial least squares discrimination analysis (PLS-DA). Pathway analysis based on PLS-DA, univariate analysis, and correlation analysis of metabolites showed that the major differential metabolites between the terrestrial N. flava and the marine ones were involved in osmotic regulation, redox balancing, and energy metabolism. Together, these insights provide clues as to how the previous living environment of microbes affect their current metabolic performances under laboratory cultivation conditions. |
| Institute | Third Institute of Oceanography, State Oceanic Administration |
| Last Name | Xia |
| First Name | Jinmei |
| Address | 184 Daxue Road, Xiamen 361005, PR China |
| xiajinmei@tio.org.cn | |
| Phone | 86-13003995626 |
| Submit Date | 2017-08-12 |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2020-01-06 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001168 |
| Collection Summary: | The broth cultures (50 mL) were harvested via centrifugation at 7000 g for 10 min under 4 °C. The pellet was quenched using 10 mL of 60% cold methanol (−80 °C) containing 0.85% (wt./vol.) of NaCl for 30 min. The quenched cell pellets were re-suspended in 10 mL of cold PBS and were washed for 3 times. |
| Sample Type: | Cultured cells |
