Summary of Study ST000948
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000652. The data can be accessed directly via it's Project DOI: 10.21228/M8D38P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000948 |
| Study Title | Mechanisms for Insulin Resistance in Polycystic Ovary Syndrome: aminoadipic acid, lysine concentrations, and enrichment (part II) |
| Study Summary | To determine how metformin therapy changes lysine and AAA kinetics in PCOS and whether this is associated with improvements in insulin sensitivity. Changes in lysine and AAA flux before and after three months of metformin therapy will be compared to women with PCOS randomized to no treatment for three months. |
| Institute | Mayo Clinic |
| Last Name | Chang |
| First Name | Alice |
| Address | 200 First St. SW, Rochester, Minnesota, 55905, USA |
| Chang.Alice1@mayo.edu | |
| Phone | 507-286-0505 |
| Submit Date | 2018-04-09 |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-04-13 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO000981 |
| Collection Summary: | We will perform a hyperinsulinemic-euglycemic clamp as previously described except that in this current proposal we will perform the study for 3 hours.(36) The goal for this infusion is to assess the effect of hyperinsulinemia on lysine and AAA kinetics and whether metformin changes the effect of hyperinsulinemia. We will collect samples every 10 min for glucose. 40% dextrose will be infused at a variable rate during the clamp to maintain euglycemia.(36) During the last hour of the clamp, 5 blood samples will be drawn for measurement of stable isotope tracer enrichment and amino acid concentrations. Previous studies have established that the combination of metformin and pioglitazone decrease lysine and AAA concentrations in overweight and obese adults with impaired fasting glucose or untreated diabetes.(7) Aim 3 is designed to establish whether changes in lysine and AAA flux are associated with changes in insulin sensitivity. Measurement of lysine and AAA flux will be repeated in 10 women with PCOS after three months of metformin and 10 women with PCOS randomized to receive no treatment for three months. Insulin sensitivity and other measures of glucose metabolism will be calculated from an oral glucose tolerance test using the oral minimal model as described below. |
| Sample Type: | Blood (plasma) |
