Summary of Study ST001086

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000725. The data can be accessed directly via it's Project DOI: 10.21228/M8ZM3X This work is supported by NIH grant, U2C- DK119886.

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Study IDST001086
Study TitleTargeted GC-MS of SETD2 isogenic cell lines
Study SummaryIn this study, SETD2 null isogenic 38E/38F clones derived from 786-O cells were generated by zinc finger nucleases, and the cellular metabolic changes of 786-O (WT) and 38E/38F isogenic cell lines (n=3 per group) were analyzed by GC-MS-based targeted metabolomics.
Institute
Arizona State University
DepartmentCollege of Health Solutions
Last NameLiu
First NameJingping
Address13208 E. Shea Blvd, Scottsdale, AZ
Emailjliu381@asu.edu
Phone4802078529
Submit Date2018-10-24
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2018-12-11
Release Version1
Jingping Liu Jingping Liu
https://dx.doi.org/10.21228/M8ZM3X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001124
Collection Summary:Quickly aspirate cell culture medium from 3.5 cm plates, gently dispense 2 mL of PBS to rinse cells, shake the plate back and forth for 2 s and then quickly remove the PBS. Immediately add 1 mL 8:2 methanol:H2O (on dry ice) into the plates placed on dry ice to quench cell metabolism, store samples at -80℃.
Sample Type:Cultured cells
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