Summary of Study ST001265

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000850. The data can be accessed directly via it's Project DOI: 10.21228/M8T98G This work is supported by NIH grant, U2C- DK119886.


Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST001265
Study TitleComparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy
Study TypeAnalysing metabolomics using GC Mass Spectroscopy
Study SummaryMetabolic profiling of cancer cells can play a vital role in revealing the molecular bases of cancer development and progression. In this study, gas chromatography coupled with mass spectrometry (GC-MS) was employed for the determination of signatures found in ER+/ PR+ breast cancer cells derived from MCF-7 using different extraction solvents including: A, formic acid in water; B, ammonium hydroxide in water; C, ethyl acetate; D, methanol: water (1:1, v/v); and E, acetonitrile: water (1:1, v/v). The greatest extraction rate and diversity of metabolites occurs with extraction solvents A and E. Extraction solvent D showed moderate extraction efficiency, whereas extraction solvent B and C showed inferior metabolite diversity. Metabolite set enrichment analysis results showed energy production pathways to be key in MCF-7 cell lines. This study showed that mass spectrometry could identify key metabolites associated with cancers. The highest enriched pathways were related to energy production as well as Warburg effect pathways, which may shed light on how energy metabolism has been hijacked to encourage tumour progression and eventually metastasis in breast cancer.
Sharjah Institute for Medical Research
DepartmentClinical Science
Last NameHamoudi
First NameRifat
AddressCollege of Medicine, University of Sharjah
Submit Date2019-07-08
Total SubjectsFive different extractions
Study CommentsMCF-7 cell line
Raw Data AvailableYes
Raw Data File Type(s)gqd
Analysis Type DetailGC-MS
Release Date2019-10-11
Release Version1
Rifat Hamoudi Rifat Hamoudi application/zip

Select appropriate tab below to view additional metadata details:


Collection ID:CO001327
Collection Summary:The breast adenocarcinoma cells (MCF-7) were cultured in DMEM medium supplemented with 10% fetal calf serum and 1% penicillin/streptomycin solution and incubated at 37oC in an atmosphere of 5% CO2. In preparation for an experiment, 3 × 106 cells were cultured in each of 15 tissue culture flasks (175 cm2) and cells were incubated for three days. When the cells reach 80% confluency, they were collected by trypsinization, counted in a cell counter and each 3 ×106 cells were suspended in 1 ml phosphate-buffered saline in an Eppendorf tube.
Sample Type:Breast cancer cells
Collection Method:trypsinization protocol
Storage Conditions:-80℃