Summary of Study ST001280
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000865. The data can be accessed directly via it's Project DOI: 10.21228/M8W40B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001280 |
Study Title | Macrophage-Mediated Clofazimine Sequestration is Accompanied by a Shift in Host Energy Metabolism |
Study Type | 4 timepoints for urine collection, whole blood collected at sacrifice (8 weeks) |
Study Summary | Examined the effects of long-term dosing with CFZ of mice, metabolic data on CFZ and sham mice was collected at 0, 2, 4, and 8 weeks, at 8 weeks mice were sacrificed and WB was collected from 6 CFZ treated mice and 7 sham mice |
Institute | University of Michigan |
Department | Clinical Pharmacy |
Laboratory | NMR Metabolomics Laboratory, University of Michigan |
Last Name | Stringer |
First Name | Kathleen |
Address | College of Pharmacy, 428 Church St, Ann Arbor, MI, 48109 |
NMRmetabolomics@umich.edu | |
Phone | 734-647-4775 |
Submit Date | 2019-11-05 |
Num Groups | 2 |
Total Subjects | 13, 48 urine Samples, 13 WB Samples |
Num Males | 13 |
Publications | 10.1016/j.xphs.2016.12.009 |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2020-01-13 |
Release Version | 1 |
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Collection:
Collection ID: | CO001346 |
Collection Summary: | Urine: 18 hour collection from metabolic cages. At the end of each collection urine volume was measured and centrifuged (2000xg, 10 mins, °C). Internal standard was added equivalent to 10% of sample volume, aliquots were transferred into screw-top cryotubes and stored at -80°C. WB: cardiac puncture after anesthesia. Collected by cardiac puncture and transferred to a microcentrifuge tube containing sodium heparin in an ice water bath. Stored @ -80°C within 10 minutes of collection |
Collection Protocol Filename: | MouseUrineCollection,Processing_MetabolicCages.V1.5.pdf |
Collection Protocol Comments: | Urine Collected 4 times over 8 weeks, each collection over 18 hours |
Sample Type: | Urine |
Collection Method: | From Metabolic cages |
Collection Frequency: | 2 weeks |
Collection Duration: | 18hr |
Storage Conditions: | -80℃ |
Collection Vials: | Sterile 2mL microcentrifuge tubes |
Storage Vials: | Sterile 2mL microcentrifuge tubes |
Collection Tube Temp: | Room temp |