Summary of Study ST001878
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001184. The data can be accessed directly via it's Project DOI: 10.21228/M8NX2V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001878 |
| Study Title | Targeted analysis of Babesia divergens merozoites |
| Study Summary | The study comprehends two consecutive LC-QqQ/MS analyses of Babesia divergens merozoite extracts isolated from B. divergens infected red blood cell cultures performed under identical chromatographic conditions and targeting distinct transitions corresponding to metabolites from specific pathways including the glycolysis, the TCA cycle, the pentose phosphate pathway, purine and pyrimidine biosynthesis and amino acid metabolism. |
| Institute | Universidad CEU San Pablo |
| Department | Departamento de Quimica y Bioquimica |
| Laboratory | Centro de Metabolomica y Bioanalisis (CEMBIO) |
| Last Name | Fernandez |
| First Name | Miguel |
| Address | Universidad CEU San Pablo |
| mig.fernandez.ce@ceindo.ceu.es | |
| Phone | 690090778 |
| Submit Date | 2020-12-16 |
| Num Groups | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-07-30 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001948 |
| Collection Summary: | Merozoites were isolated from B. divergens infected erythrocite (iRBC) cultures at 40% parasitemia. The content from iRBC culture flasks was transferred to Falcon tubes and spinned at 600 x g and 4 ºC for 5 min. Then, supernatants were filtered once using 5 μm filters and twice using 1.2 μm filters (Versapor membranes) with the help of a 1 mL syringe. Subsequently, the resulting volume was spinned on a Falcon tube at 2000 x g and 4 ºC for 2 min. The supernatants were discarded, and merozoite-containing pellets were placed on ice. |
| Sample Type: | merozoites isolated from RBC |
