Summary of Study ST002195
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001399. The data can be accessed directly via it's Project DOI: 10.21228/M8WB0D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002195 |
| Study Title | Untargeted lipidomics studies in the course of dermatitis onset and progression |
| Study Summary | We applied untargeted lipidomic analysis to the atopic dermatitis-like dermatitis model (Spade mice) to capture the comprehensive lipidome profile in the course of dermatitis onset and progression. Spade mice harbor a single amino acid mutation in Jak1 that causes hyperactivation, leading to Th2 dermatitis. Progressive dermatitis develops as desquamation and redness of the ears at approximately 8 weeks of age. At 10 weeks of age, serum IgE and IgG1 levels are increased, and Th2 cytokines, such as IL-4, IL-5, and IL-13, produced by CD4+ cells are upregulated, followed by elevated serum histamine levels at 12 weeks of age. Skin lesions manifest as epidermal hyperplasia at 8 weeks of age, while there are few morphological changes at 4 weeks of age. TEWL, the readout for barrier function, is significantly elevated in Spade mice at 4 weeks of age, suggesting that barrier defects had occurred before disease onset. WT and Spade skin tissues in P0, 4, 8, and 10 weeks of age were applied to untargeted lipidomics. Over 700 skin lipids including glycerophospholipids, ceramides, neutral lipids, and fatty acids were successfully annotated, and many of them were found to be significantly changed after dermatitis onset as determined by pruritus and erythema. Among them, the levels of Cer[NdS] containing very long-chain (C22 or more) fatty acids were significantly downregulated before AD onset. |
| Institute | Graduate School of Pharmaceutical Sciences, Keio University |
| Last Name | Iino |
| First Name | Yudai |
| Address | 1-5-30, Shibakoen, Minato-ku, Tokyo, 105-8512, Japan |
| iino-iino@keio.jp | |
| Phone | 81-3-2400-5492 |
| Submit Date | 2022-05-25 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-12-12 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002274 |
| Collection Summary: | Mice were euthanized. Then, ears were collected and stored at -80˚C after rapid freezing in liquid nitrogen. |
| Sample Type: | skin |
