Summary of Study ST002232
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001422. The data can be accessed directly via it's Project DOI: 10.21228/M8X411 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002232 |
| Study Title | Steady-state metabolomics Saccharomyces cerevisiae mitochondrial fatty acid synthesis (mtFAS) mutants and CTP1 overexpression |
| Study Type | Steady-state targeted and untargeted metabolomics |
| Study Summary | The goal of this work was to analyze metabolic changes in yeast with the mct1 gene knock-out or CTP1 overexpression conditions using liquid chromatography-mass spectrometry (LC-MS). |
| Institute | University of Utah |
| Department | Biochemistry |
| Laboratory | Rutter |
| Last Name | Berg |
| First Name | Jordan |
| Address | 15 N Medical Drive East RM 5520, Salt Lake City, UT 84112-5650 USA |
| jordan.berg@biochem.utah.edu | |
| Phone | +1 (801) 581 3340 |
| Submit Date | 2022-07-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-08-08 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002311 |
| Collection Summary: | Metabolomics data were generated by growing the appropriate yeast strains in synthetic complete media supplemented with 2% glucose until they reached saturation (n=3). Cells were then transferred to S-minimal media containing 2% raffinose and leucine and harvested after approximately 8 hours (n=3) at OD600=0.6-0.8. The procedures for metabolite extraction were performed as previously described in [Bricker et al., Science, 2012]. Yeast cultures were pelleted, snap-frozen and kept at −80°C. 5ml of 75% boiled ethanol was added to every frozen pellet. Pellets were vortexed and incubated at 90°C for 5 minutes. All samples were then centrifuged at 5,000 Relative Centrifugal Force (RCF) for 10 minutes. Supernatants were transferred to fresh tubes, evaporated overnight in a Speed Vacuum, and then stored at −80°C until they were run on the mass spectrometer. |
| Sample Type: | Yeast cells |
