Summary of Study ST002320
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001486. The data can be accessed directly via it's Project DOI: 10.21228/M8N999 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002320 |
| Study Title | Untargeted Fecal Metabolomic Analyses Across an Industrialization Gradient Reveal Shared Metabolites and Impact of Industrialization on Fecal Microbiome-Metabolome Interactions |
| Study Summary | The metabolome is a central determinant of human phenotypes and includes the plethora of small molecules produced by host and microbiome, or taken up from exogenous sources. However, studies of the metabolome have so far focused predominantly on urban, industrialized populations. Through an untargeted metabolomic analysis of 90 fecal samples from human individuals from Africa and the Americas—the birthplace and the last continental expansion of our species, respectively—we characterized a shared human fecal metabolome. The majority of detected metabolite features were ubiquitous across populations, despite any geographic, dietary, or behavioral differences. Such shared metabolite features included hyocholic acid and cholesterol. However, any characterization of the shared human fecal metabolome is insufficient without exploring the influence of industrialization. Here, we show chemical differences along an industrialization gradient, where the degree of industrialization correlates with metabolomic changes. We identified differential metabolite features like amino acid-conjugated bile acids and urobilin as major metabolic correlates of these behavioral shifts. Additionally, co-analyses with over 5,000 publicly available human fecal samples and co-occurrence probability analyses with the gut microbiome highlight connections between the human fecal metabolome and gut microbiome. Our results indicate that industrialization significantly influences the human fecal metabolome, but diverse human lifestyles and behavior still maintain a shared human fecal metabolome. This study represents the first characterization of the shared human fecal metabolome through untargeted analyses of populations along an industrialization gradient. |
| Institute | University of Oklahoma |
| Last Name | Haffner |
| First Name | Jacob |
| Address | 101 David L. Boren Blvd, Norman, OK, 73019 |
| jacob.haffner@ou.edu | |
| Phone | 405-325-7381 |
| Submit Date | 2022-10-07 |
| Num Groups | 6 |
| Total Subjects | 90 |
| Num Males | 29 |
| Num Females | 47 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-10-25 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002399 |
| Collection Summary: | Fecal material was deposited into polypropylene containers and then put on ice. Samples were kept in ice while in the field until arriving at research facilities equipped with freezers. The Norman samples were kept in ice after collection and frozen at the laboratory within 24 hours. The Peruvian samples were secured similarly to the Norman samples. After collection, samples were stored on ice for four days until arriving at Lima, Peru. Samples were frozen and sent to the laboratory in Norman, Oklahoma. Boulkiemdé samples were collected similarly to Norman and Peruvian samples. After collection, Boulkiemdé samples were frozen at -20 °C within 24 hours and kept frozen overnight. Samples were thawed the following evening to extract DNA, refrozen at -20 °C, and kept frozen until shipped to the laboratory in Norman, Oklahoma. Upon arrival, 2 g of fecal material was extracted from each sample for anaerobic culturing. Following this 2 g aliquoting, samples were frozen at -80 °C until use for this project. The Norman, Tunapuco, and Matses samples had previously been aliquoted and underwent 16S rRNA gene sequencing for an earlier study. |
| Sample Type: | Feces |
| Collection Location: | United States; Peru; Burkina Faso |
| Volumeoramount Collected: | Variable; 50mg used for experimental analyses |
| Storage Conditions: | -80℃ |
