Summary of Study ST002363

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001518. The data can be accessed directly via it's Project DOI: 10.21228/M8GH72 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002363
Study Title[U-13C]glucose tracing in NT, AOA or EGCG treated activated CD8+ T cells
Study SummaryCD8+ T cells were activated with plate-bound anti-CD3 and soluble anti-CD28 for 24 hours without (NT), or with AOA (250uM) or EGCG (500uM) treatment. CD8+ T cells were pulsed with [U-13C]glucose. Intracellular glucose-derived glutamate levels were quantified using MS.
Institute
Johns Hopkins University
Last NameXu
First NameWei
Address1650 Orleans Street, Baltimore, MD 21287, USA.
Emailwxu29@jhmi.edu
Phone443-220-9936
Submit Date2022-11-28
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2022-12-15
Release Version1
Wei Xu Wei Xu
https://dx.doi.org/10.21228/M8GH72
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO002445
Collection Summary:Cells were spun down and washed once with pre-warmed PBS and metabolites were immediately extracted or stored at -80℃ until further extraction.
Sample Type:Cultured cells
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