Summary of Study ST002458
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001585. The data can be accessed directly via it's Project DOI: 10.21228/M8TD8H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002458 |
| Study Title | Mouse kidney metabolomics (Proximal tubular cells) |
| Study Type | MS quantitative analysis |
| Study Summary | Here, we reveal for the first time the misregulated metabolic pathways in TSC kidneys and their relevance to TSC-associated cytogenesis. To this end, we have analyzed the metabolic profile of the whole kidney as well as sorted proximal tubule cell (PTCs) extracts. The metabolomics data show that Tsc1 deletion in nephron progenitor cells changes the arginine biosynthesis pathway as well as a substantial number of metabolic pathways. |
| Institute | Hadassah Medical Center |
| Last Name | Ben-Dov |
| First Name | Iddo |
| Address | Hadassah Medical Center, Jerusalem, Israel 91120 |
| iddo@hadassah.org.il | |
| Phone | +97226776881 |
| Submit Date | 2023-01-31 |
| Num Groups | 3 |
| Total Subjects | 26 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-02-26 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002541 |
| Collection Summary: | PTCs were isolated as previously described(PMID: 32484794). Briefly, kidneys were excised in ice-cold HBSS buffer. The kidneys were sliced and chopped into pieces (~0.5–1 mm) on ice using a surgical scalpel. The chopped kidneys were transferred into an HBSS solution containing 1 μg/μL collagenase/dispase (Sigma Aldrich, Cat. # 10269638001) and incubated for 25 minutes at 370 C. The cells were filtered through a 40-μm nylon cell strainer (Corning, Cat. # 431750) and washed twice with cold HBSS. For PTC isolation, the cells were stained with PE-conjugated antiCD133/prominin-1 antibody (Invitrogen, Cat. # 12-1331-82) according to the manufacturer's instructions. PE+ cells were isolated by Aria III flow cytometry-based cell sorting. |
| Sample Type: | Proximal tubular cells |
