Summary of Study ST002701
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001672. The data can be accessed directly via it's Project DOI: 10.21228/M8KH8C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002701 |
| Study Title | Mouse serum metabolomics |
| Study Summary | The brain and gut are intricately connected in response to various stressful stimuli. Here we describe a brain-to-gut pathway in mice that instructs intestinal stem cells (ISCs) lineage commitment via bacterial metabolic signals. Psychological stress signals from the brain trigger a sympathetic pathway to enrich gut commensal Lactobacillus, which contributes to a transferrable loss of intestinal secretory cell subtypes. Indole-3-acetate (IAA) production by Lactobacillus murinus disrupts mitochondrial bioenergetics of ISCs and blocks secretory lineage commitment. In patients with mental stress, we observe similar enrichment of IAA and Lactobacillus species associated with gut dysfunction. These findings uncover a stress-responsive brain-gut signalling mechanism that skews ISCs fate decision and could be targeted for stress-driven gut-brain comorbidities. |
| Institute | China Pharmaceutical University |
| Last Name | yuanlong |
| First Name | hou |
| Address | nanjing |
| jian2103@163.com | |
| Phone | 18851105337 |
| Submit Date | 2023-05-12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-05-26 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002799 |
| Collection Summary: | To precipitate proteins, we combined 100 μl of SERUM with 800 μl of methanol, incubated at -20 for 30 min, then centrifuged at 18000 rpm for 10 min. Subsequently, 850 μl of supernatant was transferred to a new tube and evaporated to dryness in a Thermo Savant SpeedVac. Then 200 μl of resuspension solvent methanol was added, compounds were dissolved by vortexing for 5 min and samples were clarified by centrifuged at 18000 rpm for 10 min. |
| Sample Type: | Blood (serum) |
