Summary of Study ST002870
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001793. The data can be accessed directly via it's Project DOI: 10.21228/M8Z433 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002870 |
| Study Title | A carbon-nitrogen negative feedback loop underlies the repeated evolution of cnidarian-Symbiodiniaceae symbioses across >700 Myr |
| Study Summary | Symbiotic associations with Symbiodiniaceae have evolved independently across a diverse range of cnidarian taxa including reef-building corals, sea anemones, and jellyfish, yet the molecular mechanisms underlying their regulation and repeated evolution are still elusive. Here we show that despite their independent evolution, cnidarian hosts employ the same carbon-nitrogen negative feedback loop to control symbiont proliferation. Symbiont-derived photosynthates are used to assimilate nitrogenous waste via GS/GOGAT mediated amino acid biosynthesis in a carbon dependent manner, which regulates the availability of nitrogen to the symbionts. Using nutrient supplementation experiments, we show that the provision of additional carbohydrates significantly reduces symbiont density while ammonium promotes symbiont proliferation. UHPLC-HR-MS analysis confirmed that all hosts co-incorporated glucose-derived 13C and ammonium-derived 15N via GS/GOGAT mediated amino acid biosynthesis. Our results reveal a general carbon-nitrogen negative feedback loop underlying these symbioses and provide a parsimonious explanation for their repeated evolution. |
| Institute | King Abdullah University of Science and Technology |
| Last Name | Cui |
| First Name | Guoxin |
| Address | 4700 KAUST, 23955-6900 Thuwal |
| guoxin.cui@kaust.edu.sa | |
| Phone | +966544701749 |
| Submit Date | 2023-09-12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-10-04 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002975 |
| Collection Summary: | Coral fragments and jellyfish were collected from the Red Sea and maintained in the lab for more than 6 months before the experiment. Sea anemone Exaiptasia were maintained in the lab for more than 5 years before the experiment. |
| Sample Type: | Whole invertebrate animal |
