Summary of Study ST002911
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001811. The data can be accessed directly via it's Project DOI: 10.21228/M8MQ7C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002911 |
| Study Title | LiLA: Lipid Lung-based ATLAS built Through a Comprehensive Workflow Designed for an Accurate Lipid Annotation |
| Study Summary | Accurate lipid annotation is crucial for understanding the role of lipids in health and disease and identifying therapeutic targets. However, annotating the wide variety of lipid species in biological samples remains challenging in untargeted lipidomic studies. In this work, we present an optimized lipid annotation workflow based on the combination of LC-MS and MS/MS strategies, four bioinformatic tools, and a decision-tree-based approach to support the accurate annotation and semi-quantification of the lipid species present in lung tissue from control mice. The developed workflow allowed us to generate a lipid lung-based ATLAS (LiLA), which was then employed to unveil the lipidomic signatures of the Mycobacterium tuberculosis (Mtb) infection at two different time points for a deeper understanding of the disease progression. This workflow, combined with manual inspection strategies of MS/MS data, can enhance the annotation process for lipidomic studies and guide the generation of sample-specific lipidome maps. LiLA serves as a freely available data resource that can be employed in future studies to address lipidomic alterations in mice lung tissue. |
| Institute | Universidad CEU San Pablo |
| Department | Centro de MEtabolómica y Bioanálisis (CEMBIO) |
| Last Name | González |
| First Name | Carolina |
| Address | km 0, Universidad CEU-San Pablo Urbanización Montepríncipe. M-501 |
| carolina.gonzalezriano@ceu.es | |
| Phone | 646251045 |
| Submit Date | 2023-10-02 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-10-20 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003017 |
| Collection Summary: | Animals were divided into three different groups: healthy controls (Mtb-, n=6), infected mice four weeks post-infection (Mtb+4w, n=6) and infected mice twelve weeks post-infection (Mtb+12w, n=5). Seven- to eight-week-old female BALB/c mice were purchased from Jackson Laboratories. Mice were housed in a pathogen-free facility with ad libitum access to water and food. All the animal studies were performed following the guidelines approved by IACUC at the University of Alabama at Birmingham, USA. Mice were aerosol infected with Mtb H37Rv using the aerosol inhalation exposure system (Glas-Col, USA) to deliver ~120-250 CFU/mouse lung. The infection dose was estimated by enumerating the lung CFU at 24 hours post-infection. Mice were sacrificed using anaesthesia with isoflurane followed by gentle cervical dislocation. Mice organs were aseptically harvested and homogenized in 2 ml of 1x PBS, pH 7.4. Serial dilutions of homogenates were prepared in 1x PBS and plated on 7H11 agar plates supplemented with 10% ADS (Albumin, Dextrose, and NaCl), Carbenicillin (25 mg/L) and Cycloheximide (25 mg/L). Plates were incubated at 37 °C for ~21 days before counting colonies. Finally, lung samples were transferred to an N2(l)-containing recipient to freeze the tissues and stored at -80 °C to avoid postmortem metabolic processes. |
| Sample Type: | Lung |
