Summary of study ST000145

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000145
Study TitlePrimary T Cell Noxa Knockdown (Donor 8)
Study SummaryCells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 12 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-03-13
Num Groups3
Total Subjects15
Raw Data AvailableYes
Raw Data File Type(s).cd,.cG, .xml, .bin,.stg
Uploaded File Size3.8 G
Analysis Type DetailGC/LC-MS
Release Date2015-03-13
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M85C7W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Label
SA008028S00016916Glucose
SA008029S00016929Glucose
SA008030S00016921Glucose
SA008031S00016920Glucose
SA008032S00016918Glucose
SA008033S00016919Glucose
SA008034S00016917Glucose
SA008035S00016928Glutamine
SA008036S00016930Glutamine
SA008037S00016927Glutamine
SA008038S00016923Glutamine
SA008039S00016922Glutamine
SA008040S00016924Glutamine
SA008041S00016925Glutamine
SA008042S00016926Glutamine
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