Summary of Study ST000170

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000223. The data can be accessed directly via it's Project DOI: 10.21228/M8PW2T This work is supported by NIH grant, U2C- DK119886.


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Study IDST000170
Study Title13C mass isotopomer analysis (LCMS flux studies) MLL-AF9 (part III)
Study TypeGlycolysis/TCA/Nucleotide analysis (tissue/cells)
Study SummaryHow cancer cells adapt to metabolically adverse conditions in patients and strive to proliferate is a fundamental question in cancer biology. Here we show that AMP-activated protein kinase (AMPK), a metabolic checkpoint kinase, confers metabolic stress resistance to leukemia-initiating cells (LICs) and promotes leukemogenesis. Upon dietary restriction, MLL-AF9-induced murine acute myeloid leukemia (AML) activated AMPK and maintained leukemogenic potential. AMPK deletion significantly delayed leukemogenesis and depleted LICs by reducing the expression of glucose transporter 1 (Glut1), compromising glucose flux, and increasing oxidative stress and DNA damage. LICs were particularly dependent on AMPK to suppress oxidative stress in the hypoglycemic bone marrow environment. Strikingly, AMPK inhibition synergized with physiological metabolic stress caused by dietary restriction and profoundly suppressed leukemogenesis. Our results indicate that AMPK protects LICs from metabolic stress and that combining AMPK inhibition with physiological metabolic stress potently suppresses AML by inducing oxidative stress and DNA damage.
University of Michigan
DepartmentMolecular and Human genetics (Baylor College of Medicine)
LaboratoryNakada Lab
Last NameSaitoh
First NameYusuke
AddressHouston, TX>
Submit Date2015-01-13
Num Groups1
Total Subjects9
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-01-13
Release Version1
Yusuke Saitoh Yusuke Saitoh application/zip

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Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id GENOTYPE GROUP
SA012282S00017008AMPKαfl/fl Bone marrow
SA012283S00017012AMPKαfl/fl Bone marrow
SA012284S00017010AMPKαfl/fl Bone marrow
SA012285S00017009AMPKαfl/fl Spleen
SA012286S00017011AMPKαfl/fl Spleen
SA012287S00017013AMPKαfl/fl Spleen
SA012279S00017014AMPKαΔ/Δ Bone marrow
SA012280S00017015AMPKαΔ/Δ Bone marrow
SA012281S00017016AMPKαΔ/Δ Bone marrow
Showing results 1 to 9 of 9