Summary of Study ST000701

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000148. The data can be accessed directly via it's Project DOI: 10.21228/M8V011 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000701
Study TitleDifferences in metabolism in mouse embryonic fibroblasts (control vs SCF-beta-TrCP knockout)
Study TypeMS analysis
Study SummaryMouse embryonic fibroblasts were grown in DMED supplemented with 10%FBS. The same number of cells were plated in eight 6cm dishes. When the cells were ~70% confluent, four dishes received ethanol (carrier) and the other four received tamoxifen (1uM final) to induce knockout. The cells and media were harvested 4 days later. The media harvested from control and knockout dishes will be compared with the starting medium. The cells were washed with cold PBS twice while they are attached to the dishes. The whole dish with cells attached were immediately frozen in dry ice and wrapped with aluminum foil and stored at -80C.
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups6
Total Subjects3
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman application/zip

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Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id GENOTYPE Sample type
SA039526S00013520Control Fibroblasts
SA039527S00013519Control Fibroblasts
SA039528S00013521Control Fibroblasts
SA039529S00013518Control Fibroblasts
SA039530S00013515Control Fresh media
SA039531S00013516Control Spent media
SA039532S00013525Knockout Fibroblasts
SA039533S00013524Knockout Fibroblasts
SA039534S00013523Knockout Fibroblasts
SA039535S00013522Knockout Fibroblasts
SA039536S00013517Knockout Spent media
SA039537S00020125Pooled Fibroblasts
SA039538S00020124Pooled Spent media
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