Summary of Study ST001035

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000693. The data can be accessed directly via it's Project DOI: 10.21228/M83H4H This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001035
Study Title	TCA Isotopomers in Neuromyelitis Optica Patients (part - I)
Study Summary	Patients with an inflammatory disease of the central nervous system known as neuromyelitis optica (NMO) experience increased levels of depression. These patients have an antibody that recognizes a type of cell in the brain called astrocytes – binding of this antibody to astrocytes triggers a stress response in the cell that results in the development of brain lesions that cause disability and cognitive disturbances. We recently observed a change in the level of glutamate in a part of the brain involved in depression in patients with NMO. Glutamate is a chemical that is used in the brain for communication between neurons – reduced levels of glutamate are thought to trigger depression by reducing neuronal activity in specific circuits. Based on this observation and the known role of astrocytes in maintaining glutamate levels in the brain, we hypothesize that the NMO antibody disturbs metabolic activity in astrocytes and thereby reduces glutamate and triggers depression. We intend to trace the metabolic response induced in astrocytes by the NMO antibody using TCA isotopomers. It is our hope that we will not only learn something about the mechanisms of astrocyte dysregulation in neuromyelitis optica, but that we will learn something about the mechanisms of depression in general that may lead to new therapies for this disease.
Institute	Mayo Clinic
Last Name	Howe
First Name	Charles
Address	200 First St. SW, Rochester, Minnesota, 55905, USA
Email	howe@mayo.edu
Phone	507-284-9288
Submit Date	2018-08-13
Analysis Type Detail	GC-MS
Release Date	2020-08-20
Release Version	1

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Time	condition	matrix	Tracer
SA069228	ms6905-32	0h	BGP 50uMGt Gn	Fresh Media	Unlabelled
SA069230	ms6905-30	0h	BGPGt 0.5mM Gn	Fresh Media	Unlabelled
SA069229	ms6983-32	0h	BGPGt 0.5 mM Gn	Fresh med	Unlabelled
SA069232	ms6983-34	0h	BGPGt 50 mM Gn	Fresh med	Unlabelled
SA069233	ms6905-31	0h	BGPGt 5mM Gn	Fresh Media	Unlabelled
SA069231	ms6983-33	0h	BGPGt 5 mM Gn	Fresh med	Unlabelled
SA069234	ms6905-25	0h	E Med	Fresh Media	13C6
SA069235	ms6983-25	0h	E Med	Fresh med	13C6
SA069236	ms6983-35	0h	E Med	Fresh med	Unlabelled
SA069223	MS6735-41	0 hr	BGPGt - 0.5mM Gn	Fresh med	Unlabelled
SA069224	MS6735-40	0 hr	BGPGt - 0.5mM Gn	Fresh med	Unlabelled
SA069225	MS6735-39	0 hr	BGPGt	Fresh med	Unlabelled
SA069226	MS6735-26	0 hr	E Med	Fresh med	13C6
SA069227	MS6735-25	0 hr	E Med	Fresh med	Unlabelled
SA069238	ms6905-22	1h nonCO2	BGP50uMGt Gn	Spent Sup	13C6
SA069239	ms6905-24	1h nonCO2	BGP50uMGt Gn	Spent Sup	13C6
SA069240	ms6905-23	1h nonCO2	BGP50uMGt Gn	Spent Sup	13C6
SA069237	ms6905-21	1h nonCO2	BGP 50uMGt Gn	Spent Sup	Unlabelled
SA069241	MS6735-19	1h nonCO2	BGPGt - 0.5mM Gn	Spent Sup	13C6
SA069242	MS6735-20	1h nonCO2	BGPGt - 0.5mM Gn	Spent Sup	13C6
SA069243	MS6735-18	1h nonCO2	BGPGt - 0.5mM Gn	Spent Sup	13C6
SA069249	ms6983-16	1h nonCO2	BGPGt 0.5 mM Gn	Spent Sup	13C6
SA069250	ms6983-15	1h nonCO2	BGPGt 0.5 mM Gn	Spent Sup	13C6
SA069251	ms6983-14	1h nonCO2	BGPGt 0.5 mM Gn	Spent Sup	13C6
SA069253	ms6905-15	1h nonCO2	BGPGt 0.5mM Gn	Spent Sup	13C6
SA069254	ms6905-16	1h nonCO2	BGPGt 0.5mM Gn	Spent Sup	13C6
SA069255	ms6905-14	1h nonCO2	BGPGt 0.5mM Gn	Spent Sup	13C6
SA069244	MS6735-17	1h nonCO2	BGPGt - 0.5mM Gn	Spent Sup	Unlabelled
SA069252	ms6983-13	1h nonCO2	BGPGt 0.5 mM Gn	Spent Sup	Unlabelled
SA069256	ms6905-13	1h nonCO2	BGPGt 0.5mM Gn	Spent Sup	Unlabelled
SA069261	ms6983-22	1h nonCO2	BGPGt 50 mM Gn	Spent Sup	13C6
SA069262	ms6983-24	1h nonCO2	BGPGt 50 mM Gn	Spent Sup	13C6
SA069263	ms6983-23	1h nonCO2	BGPGt 50 mM Gn	Spent Sup	13C6
SA069264	ms6983-21	1h nonCO2	BGPGt 50 mM Gn	Spent Sup	Unlabelled
SA069245	MS6735-22	1h nonCO2	BGPGt - 5mM Gn	Spent Sup	13C6
SA069246	MS6735-24	1h nonCO2	BGPGt - 5mM Gn	Spent Sup	13C6
SA069247	MS6735-23	1h nonCO2	BGPGt - 5mM Gn	Spent Sup	13C6
SA069257	ms6983-18	1h nonCO2	BGPGt 5 mM Gn	Spent Sup	13C6
SA069258	ms6983-20	1h nonCO2	BGPGt 5 mM Gn	Spent Sup	13C6
SA069259	ms6983-19	1h nonCO2	BGPGt 5 mM Gn	Spent Sup	13C6
SA069265	ms6905-20	1h nonCO2	BGPGt 5mM Gn	Spent Sup	13C6
SA069266	ms6905-19	1h nonCO2	BGPGt 5mM Gn	Spent Sup	13C6
SA069267	ms6905-18	1h nonCO2	BGPGt 5mM Gn	Spent Sup	13C6
SA069248	MS6735-21	1h nonCO2	BGPGt - 5mM Gn	Spent Sup	Unlabelled
SA069260	ms6983-17	1h nonCO2	BGPGt 5 mM Gn	Spent Sup	Unlabelled
SA069268	ms6905-17	1h nonCO2	BGPGt 5mM Gn	Spent Sup	Unlabelled
SA069269	MS6735-14	1h nonCO2	BGPGt	Spent Sup	13C6
SA069270	MS6735-16	1h nonCO2	BGPGt	Spent Sup	13C6
SA069271	MS6735-15	1h nonCO2	BGPGt	Spent Sup	13C6
SA069272	MS6735-13	1h nonCO2	BGPGt	Spent Sup	Unlabelled
SA069298	ms6905-10	23h +1h nonCO2	BGP50uMGt Gn	pellet	13C6
SA069299	ms6905-12	23h +1h nonCO2	BGP50uMGt Gn	pellet	13C6
SA069300	ms6905-11	23h +1h nonCO2	BGP50uMGt Gn	pellet	13C6
SA069297	ms6905-9	23h +1h nonCO2	BGP 50uMGt Gn	pellet	Unlabelled
SA069273	MS6735-8	23h + 1h nonCO2	BGPGt - 0.5mM Gn	pellet	13C6
SA069274	MS6735-7	23h + 1h nonCO2	BGPGt - 0.5mM Gn	pellet	13C6
SA069275	MS6735-6	23h + 1h nonCO2	BGPGt - 0.5mM Gn	pellet	13C6
SA069281	ms6983-03	23h + 1h nonCO2	BGPGt 0.5 mM Gn	pellet	13C6
SA069282	ms6983-04	23h + 1h nonCO2	BGPGt 0.5 mM Gn	pellet	13C6
SA069283	ms6983-02	23h + 1h nonCO2	BGPGt 0.5 mM Gn	pellet	13C6
SA069301	ms6905-3	23h +1h nonCO2	BGPGt 0.5mM Gn	pellet	13C6
SA069302	ms6905-2	23h +1h nonCO2	BGPGt 0.5mM Gn	pellet	13C6
SA069303	ms6905-4	23h +1h nonCO2	BGPGt 0.5mM Gn	pellet	13C6
SA069276	MS6735-5	23h + 1h nonCO2	BGPGt - 0.5mM Gn	pellet	Unlabelled
SA069284	ms6983-01	23h + 1h nonCO2	BGPGt 0.5 mM Gn	pellet	Unlabelled
SA069304	ms6905-1	23h +1h nonCO2	BGPGt 0.5mM Gn	pellet	Unlabelled
SA069289	ms6983-10	23h + 1h nonCO2	BGPGt 50 mM Gn	pellet	13C6
SA069290	ms6983-11	23h + 1h nonCO2	BGPGt 50 mM Gn	pellet	13C6
SA069291	ms6983-12	23h + 1h nonCO2	BGPGt 50 mM Gn	pellet	13C6
SA069292	ms6983-09	23h + 1h nonCO2	BGPGt 50 mM Gn	pellet	Unlabelled
SA069277	MS6735-10	23h + 1h nonCO2	BGPGt - 5mM Gn	pellet	13C6
SA069278	MS6735-12	23h + 1h nonCO2	BGPGt - 5mM Gn	pellet	13C6
SA069279	MS6735-11	23h + 1h nonCO2	BGPGt - 5mM Gn	pellet	13C6
SA069285	ms6983-06	23h + 1h nonCO2	BGPGt 5 mM Gn	pellet	13C6
SA069286	ms6983-07	23h + 1h nonCO2	BGPGt 5 mM Gn	pellet	13C6
SA069287	ms6983-08	23h + 1h nonCO2	BGPGt 5 mM Gn	pellet	13C6
SA069305	ms6905-7	23h +1h nonCO2	BGPGt 5mM Gn	pellet	13C6
SA069306	ms6905-8	23h +1h nonCO2	BGPGt 5mM Gn	pellet	13C6
SA069307	ms6905-6	23h +1h nonCO2	BGPGt 5mM Gn	pellet	13C6
SA069280	MS6735-9	23h + 1h nonCO2	BGPGt - 5mM Gn	pellet	Unlabelled
SA069288	ms6983-05	23h + 1h nonCO2	BGPGt 5 mM Gn	pellet	Unlabelled
SA069308	ms6905-5	23h +1h nonCO2	BGPGt 5mM Gn	pellet	Unlabelled
SA069293	MS6735-3	23h + 1h nonCO2	BGPGt	pellet	13C6
SA069294	MS6735-2	23h + 1h nonCO2	BGPGt	pellet	13C6
SA069295	MS6735-4	23h + 1h nonCO2	BGPGt	pellet	13C6
SA069296	MS6735-1	23h + 1h nonCO2	BGPGt	pellet	Unlabelled
SA069309	ms6905-28	23h	E Med	Spent Sup	13C6
SA069310	ms6905-29	23h	E Med	Spent Sup	13C6
SA069311	ms6905-27	23h	E Med	Spent Sup	13C6
SA069312	ms6983-29	23h	E Med	Spent Sup	13C6
SA069313	MS6735-36	23h	E Med	Spent Sup	13C6
SA069314	MS6735-37	23h	E Med	Spent Sup	13C6
SA069315	ms6983-31	23h	E Med	Spent Sup	13C6
SA069316	MS6735-38	23h	E Med	Spent Sup	13C6
SA069317	MS6735-35	23h	E Med	Spent Sup	13C6
SA069318	MS6735-34	23h	E Med	Spent Sup	13C6
SA069319	ms6983-30	23h	E Med	Spent Sup	13C6
SA069320	MS6735-33	23h	E Med	Spent Sup	13C6
SA069321	ms6905-26	23h	E Med	Spent Sup	Unlabelled
SA069322	MS6735-32	23h	E Med	Spent Sup	Unlabelled