Summary of Study ST001062

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000712. The data can be accessed directly via it's Project DOI: 10.21228/M8N965 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001062
Study TitleArabidopsis Nit1 knockout metabolomics
Study SummaryGlutathione (GSH) is a tripeptide that is implicated in various crucial physiological processes including redox buffering and protection against heavy metal toxicity. GSH is abundant in plants, with reported intracellular concentrations typically in the 1-10 millimolar range. Various aminotransferases can inadvertently transaminate the amino group of the γ-glutamyl moiety of GSH to produce deaminated glutathione (dGSH), a metabolite damage product. It was recently reported that an amidase known as Nit1 participates in dGSH breakdown in mammals and yeast. Plants have a hitherto uncharacterized homolog of the Nit1 amidase. We show that recombinant Arabidopsis Nit1 (At4g08790) has efficient amidase activity towards dGSH. Ablating the Arabidopsis Nit1 gene causes a massive accumulation of dGSH and other marked changes to the metabolome. All plant Nit1 sequences examined had predicted plastidial targeting peptides with a potential second start codon whose use would eliminate the targeting peptide. In vitro transcription/translation assays show that both potential translation start codons were used and subcellular localization of GFP fusions confirmed both cytosolic and plastidial localization. Further, we show that Arabidopsis enzymes convert GSH to dGSH at a rate of 2.8 pmol min-1 mg-1 in vitro. Our data demonstrate that plants have a dGSH repair system that is directed to at least two subcellular compartments via the use of alternative translation start sites.
Institute
University of California, Davis
Last NameFolz
First NameJacob
Address451 Health Sciences Dr., Davis, CA, 95616
Emailjfolz@ucdavis.edu
Phone7155636311
Submit Date2018-09-24
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2019-03-06
Release Version1
Jacob Folz Jacob Folz
https://dx.doi.org/10.21228/M8N965
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Plant; Subject species: Arabidopsis thaliana (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Treatment Tissue Type
SA071837A4_38Columbai-O_WT 1/2MS-media_3weeks Whole Plant
SA071838A3_37Columbai-O_WT 1/2MS-media_3weeks Whole Plant
SA071839A5_39Columbai-O_WT 1/2MS-media_3weeks Whole Plant
SA071840A6_40Columbai-O_WT 1/2MS-media_3weeks Whole Plant
SA071841A2_36Columbai-O_WT 1/2MS-media_3weeks Whole Plant
SA071842A1_35Columbai-O_WT 1/2MS-media_3weeks Whole Plant
SA071843E1_25Columbai-O_WT 1/2MS-media_3weeks Whole Plant
SA071844E5_29Columbai-O_WT 1/2MS-media_3weeks Whole Plant
SA071845E4_28Columbai-O_WT 1/2MS-media_3weeks Whole Plant
SA071846E3_27Columbai-O_WT 1/2MS-media_3weeks Whole Plant
SA071847E2_26Columbai-O_WT 1/2MS-media_3weeks Whole Plant
SA071848C5_17Columbai-O_WT 1/2MS-media_4weeks Whole Plant
SA071849C4_16Columbai-O_WT 1/2MS-media_4weeks Whole Plant
SA071850C6_18Columbai-O_WT 1/2MS-media_4weeks Whole Plant
SA071851C3_15Columbai-O_WT 1/2MS-media_4weeks Whole Plant
SA071852C1_13Columbai-O_WT 1/2MS-media_4weeks Whole Plant
SA071853C2_14Columbai-O_WT 1/2MS-media_4weeks Whole Plant
SA071854A6_6Columbai-O_WT SoilGrown_4weeks Leaves
SA071855A1_1Columbai-O_WT SoilGrown_4weeks Leaves
SA071856A5_5Columbai-O_WT SoilGrown_4weeks Leaves
SA071857A3_3Columbai-O_WT SoilGrown_4weeks Leaves
SA071858A2_2Columbai-O_WT SoilGrown_4weeks Leaves
SA071859A4_4Columbai-O_WT SoilGrown_4weeks Leaves
SA071860F3_32SALK_108849C_KO 1/2MS-media_3weeks Whole Plant
SA071861F5_34SALK_108849C_KO 1/2MS-media_3weeks Whole Plant
SA071862F4_33SALK_108849C_KO 1/2MS-media_3weeks Whole Plant
SA071863F1_30SALK_108849C_KO 1/2MS-media_3weeks Whole Plant
SA071864F2_31SALK_108849C_KO 1/2MS-media_3weeks Whole Plant
SA071865B6_46SALK_108849C_KO 1/2MS-media_3weeks Whole Plant
SA071866B1_41SALK_108849C_KO 1/2MS-media_3weeks Whole Plant
SA071867B2_42SALK_108849C_KO 1/2MS-media_3weeks Whole Plant
SA071868B3_43SALK_108849C_KO 1/2MS-media_3weeks Whole Plant
SA071869B4_44SALK_108849C_KO 1/2MS-media_3weeks Whole Plant
SA071870B5_45SALK_108849C_KO 1/2MS-media_3weeks Whole Plant
SA071871D6_24SALK_108849C_KO 1/2MS-media_4weeks Whole Plant
SA071872D5_23SALK_108849C_KO 1/2MS-media_4weeks Whole Plant
SA071873D1_19SALK_108849C_KO 1/2MS-media_4weeks Whole Plant
SA071874D2_20SALK_108849C_KO 1/2MS-media_4weeks Whole Plant
SA071875D3_21SALK_108849C_KO 1/2MS-media_4weeks Whole Plant
SA071876D4_22SALK_108849C_KO 1/2MS-media_4weeks Whole Plant
SA071877B2_8SALK_108849C_KO SoilGrown_4weeks Leaves
SA071878B1_7SALK_108849C_KO SoilGrown_4weeks Leaves
SA071879B3_9SALK_108849C_KO SoilGrown_4weeks Leaves
SA071880B6_12SALK_108849C_KO SoilGrown_4weeks Leaves
SA071881B5_11SALK_108849C_KO SoilGrown_4weeks Leaves
SA071882B4_10SALK_108849C_KO SoilGrown_4weeks Leaves
Showing results 1 to 46 of 46
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