Summary of Study ST001244

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000831. The data can be accessed directly via it's Project DOI: 10.21228/M88H5F This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001244
Study TitleUropathogenic versus Urocolonizing Escherichia coli
Study SummaryUrinary tract infections (UTIs) represent a major burden across the population, although key facets of their pathogenesis challenge physicians and investigators alike. Escherichia coli epitomizes these obstacles: this Gram-negative bacterial species is the most prevalent agent of UTIs worldwide and can also colonize the urogenital tract in a phenomenon known as asymptomatic bacteriuria (ASB). Unfortunately, at the level of the organism, the relationship between symptomatic UTI and ASB is poorly defined, confounding our understanding of microbial pathogenesis and strategies for clinical management. Unlike diarrheagenic pathotypes of E. coli, the definition of uropathogenic E. coli (UPEC) remains phenomenologic, without conserved phenotypes and (known) genetic determinants that rigorously distinguish UTI- and ASB-associated strains. This manuscript provides a cross-disciplinary review of the current issues – from interrelated mechanistic and diagnostic perspectives – and describes new opportunities by which clinical resources can be leveraged to overcome molecular challenges. Specifically, we present our work harnessing a large collection of patient-derived isolates to identify features that do (and do not) distinguish UTI- from ASB-associated E. coli strains. Analyses of biofilm formation, previously reported to be higher in ASB strains, revealed extensive phenotypic heterogeneity that did not correlate with symptomatology. However, metabolomic experiments revealed distinct signatures between ASB and cystitis isolates, including species in the purine pathway (previously shown to be critical for intracellular survival during acute infection). Together, these studies demonstrate how large-scale, wild-type approaches can help dissect the physiology of colonization-versus-infection, suggesting that the molecular definition of UPEC may rest at the level of global bacterial metabolism.
Institute
Vanderbilt University
Last NameRutledge
First NameAlexandra
Address7330 Stevenson Center Lane, NASHVILLE, TENNESSEE, 37235, USA
Emaila.rutledge@vanderbilt.edu
Phone6155046923
Submit Date2019-08-14
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2020-03-03
Release Version1
Alexandra Rutledge Alexandra Rutledge
https://dx.doi.org/10.21228/M88H5F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Bacteria; Subject species: Escherichia coli (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA090764SC_20180803_RPLCp_FMS_Sup_S3_C1ASB_1
SA090765SC_20180803_RPLCp_FMS_Sup_S2_B1ASB_1
SA090766SC_20180803_RPLCp_FMS_Sup_S1_A1ASB_1
SA090767SC_20180803_RPLCp_FMS_Sup_S8_H1Cystitis_1
SA090768SC_20180803_RPLCp_FMS_Sup_S7_G1Cystitis_1
SA090769SC_20180803_RPLCp_FMS_Sup_S6_F1Cystitis_1
SA090770SC_20180803_RPLCp_FMS_Sup_S5_E1Cystitis_1
SA090771SC_20180803_RPLCp_FMS_Sup_S9_I1Cystitis_1
SA090772SC_20180803_RPLCp_FMS_Sup_S10_J1Cystitis_1
SA090773SC_20180803_RPLCp_FMS_Sup_S12_L1Cystitis_1
SA090774SC_20180803_RPLCp_FMS_Sup_S11_K1Cystitis_1
SA090775SC_20180803_RPLCp_FMS_Sup_S4_D1Cystitis_1
SA090776SC_20180803_RPLCp_DDA2_Sup_QC_06Qcpool_1
SA090777SC_20180803_RPLCp_DDA4_Sup_QC_02Qcpool_1
SA090778SC_20180803_RPLCp_FMS_Sup_QC_05Qcpool_1
SA090779SC_20180803_RPLCp_FMS_Sup_QC_04Qcpool_1
SA090780SC_20180803_RPLCp_DDA6_Sup_QC_10Qcpool_1
SA090781SC_20180803_RPLCp_FMS_Sup_QC_07Qcpool_1
SA090782SC_20180803_RPLCp_FMS_Sup_QC_09Qcpool_1
SA090783SC_20180803_RPLCp_DDA4_Sup_QC_08Qcpool_1
SA090784SC_20180803_RPLCp_DDA2_Sup_QC_01Qcpool_1
SA090785SC_20180803_RPLCp_FMS_Sup_QC_11Qcpool_1
SA090786SC_20180803_RPLCp_DDA6_Sup_QC_03Qcpool_1
Showing results 1 to 23 of 23
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