Summary of Study ST001770

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001127. The data can be accessed directly via it's Project DOI: 10.21228/M81D6M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001770
Study TitleApplication of the redox metabolite detection method for profiling redox state following pharmacologic perturbations of redox balance in cells (part II)
Study SummaryThis study aimed to test our optimized method for detection of redox metabolites from mammalian cells upon redox stress and metabolism perturbations. Redox balance was perturbed using H2O2 and diamide, metabolism was perturbed by methotrexate or oligomycin. This experiment is an independent repeat.
Institute
Boston Children's Hospital, Harvard Medical School
DepartmentPathology
LaboratoryNaama Kanarek
Last NamePetrova
First NameBoryana
Address300 Longwood Av, Boston, MA, 2115, USA
Emailboryana.petrova@childrens.harvard.edu
Phone6173557433
Submit Date2021-04-22
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-05-17
Release Version1
Boryana Petrova Boryana Petrova
https://dx.doi.org/10.21228/M81D6M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA164403pooled sample QC c-
SA164404pooled sample QC b-
SA164405pooled sample QC a-
SA164406blank1-
SA164407pooled sample QC 1/3 dilution-
SA164408pooled sample QC d-
SA164409pooled sample QC 1/10 dilution a-
SA164410blank2-
SA164411blank4-
SA164412blank3-
SA164413pooled sample QC 1/10 dilution b-
SA1644141M K562 cells oligomycin 80ug/ml cbuffer C_Ell
SA1644151M K562 cells DMSO abuffer C_Ell
SA1644161M K562 cells DMSO bbuffer C_Ell
SA1644171M K562 cells DMSO cbuffer C_Ell
SA1644181M K562 cells oligomycin 80ug/ml bbuffer C_Ell
SA1644191M K562 cells DA 0.5mM bbuffer C_Ell
SA1644201M K562 cells DA 0.2mM abuffer C_Ell
SA1644211M K562 cells DA 0.2mM bbuffer C_Ell
SA1644221M K562 cells 1M K562 cellsbuffer C_Ell
SA1644231M K562 cells MTX 5uM bbuffer C_Ell
SA1644241M K562 cells MTX 5uM abuffer C_Ell
SA1644251M K562 cells DA 0.2mM cbuffer C_Ell
SA1644261M K562 cells oligomycin 40ug/ml abuffer C_Ell
SA1644271M K562 cells DA 0.5mM cbuffer C_Ell
SA1644281M K562 cells DA 0.5mM abuffer C_Ell
SA1644291M K562 cells oligomycin 40ug/ml cbuffer C_Ell
SA1644301M K562 cells oligomycin 40ug/ml bbuffer C_Ell
SA1644311M K562 cells oligomycin 80ug/ml abuffer C_Ell
Showing results 1 to 29 of 29
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