Summary of Study ST001771

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001127. The data can be accessed directly via it's Project DOI: 10.21228/M81D6M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001771
Study TitleApplication of the redox metabolite detection method for profiling redox state following pharmacologic perturbations of redox balance in cells (part III)
Study SummaryThis study aimed to test our optimized method for detection of redox metabolites from mammalian cells upon redox stress and metabolism perturbations. Redox balance was perturbed using H2O2 and diamide, metabolism was perturbed by methotrexate or oligomycin. This experiment is an independent repeat.
Institute
Boston Children's Hospital, Harvard Medical School
DepartmentPathology
LaboratoryNaama Kanarek
Last NamePetrova
First NameBoryana
Address300 Longwood Av, Boston, MA, 2115, USA
Emailboryana.petrova@childrens.harvard.edu
Phone6173557433
Submit Date2021-04-22
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-05-17
Release Version1
Boryana Petrova Boryana Petrova
https://dx.doi.org/10.21228/M81D6M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id buffer C_Ell
SA164432pooled sample QCd-
SA164433pooled sample QCc-
SA164434pooled sample QCb-
SA164435blank1-
SA164436pooled sample Qca-
SA164437pooled sample QC 1/10 dilutiona-
SA164438pooled sample QC 1/3 dilution-
SA164439blank2-
SA164440blank4-
SA164441blank3-
SA164442pooled sample QC 1/10 dilutionb-
SA164443blank5-
SA1644441M K562 cells oligomycin 80ug/ml cbuffer C_Ell
SA1644451M K562 cells H2O2 1mM abuffer C_Ell
SA1644461M K562 cells H2O2 1mM bbuffer C_Ell
SA1644471M K562 cells oligomycin 80ug/ml bbuffer C_Ell
SA1644481M K562 cells H2O2 1mM cbuffer C_Ell
SA1644491M K562 cells DA 0.5mMebuffer C_Ell
SA1644501M K562 cells MTX 5uMabuffer C_Ell
SA1644511M K562 cells MTX 5uMbbuffer C_Ell
SA1644521M K562 cells DMSOcbuffer C_Ell
SA1644531M K562 cells DMSObbuffer C_Ell
SA1644541M K562 cells DMSOabuffer C_Ell
SA1644551M K562 cells MTX 5uMcbuffer C_Ell
SA1644561M K562 cells DA 0.2mMabuffer C_Ell
SA1644571M K562 cells DA 0.5mMfbuffer C_Ell
SA1644581M K562 cells DA 0.5mMdbuffer C_Ell
SA1644591M K562 cells DA 0.2mMcbuffer C_Ell
SA1644601M K562 cells DA 0.2mMbbuffer C_Ell
SA1644611M K562 cells oligomycin 80ug/ml abuffer C_Ell
Showing results 1 to 30 of 30
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