Summary of Study ST001841

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001162. The data can be accessed directly via it's Project DOI: 10.21228/M8H992 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST001841
Study Title	Metabolomics of lung microdissections reveals region- and sex-specific metabolic effects of acute naphthalene exposure in mice (part II)
Study Summary	Naphthalene is a ubiquitous environmental contaminant produced by combustion of fossil fuels and is a primary constituent of both mainstream and side stream tobacco smoke. Naphthalene elicits region-specific toxicity in airway club cells through cytochrome P450 (P450)-mediated bioactivation, resulting in depletion of glutathione and subsequent cytotoxicity. While effects of naphthalene in mice have been extensively studied, few experiments have characterized global metabolomic changes in the lung. In individual lung regions, we found metabolomic changes in microdissected mouse lung conducting airways and parenchyma obtained from animals sacrificed 2, 6, and 24 hours following naphthalene treatment. Data on 577 unique identified metabolites were acquired by accurate mass spectrometry-based assays focusing on lipidomics and non-targeted metabolomics of hydrophilic compounds. Statistical analyses revealed distinct metabolite profiles between the two major lung regions. In addition, the number and magnitude of statistically significant exposure-induced changes in metabolite abundance were different between lung airways and parenchyma for unsaturated lysophosphatidylcholines (LPCs), dipeptides, purines, pyrimidines, and amino acids. Importantly, temporal changes were found to be highly distinct for male and female mice, with males exhibiting predominant treatment-specific changes only at two hours post-exposure. In females, metabolomic changes persisted until six hours post-naphthalene treatment, which may explain the previously characterized higher susceptibility of female mice to naphthalene toxicity. In both males and females, treatment-specific changes corresponding to lung remodeling, oxidative stress response, and DNA damage were observed, which may provide insights into potential mechanisms contributing to the previously reported effects of naphthalene exposure in the lung.
Institute	University of California, Davis
Department	Genome Center
Laboratory	Fiehn Lab
Last Name	Stevens
First Name	Nathanial C.
Address	451 Health Sciences Drive University of California Davis Davis, CA 95616
Email	ncstevens@ucdavis.edu
Phone	828-284-4315
Submit Date	2021-06-17
Raw Data Available	Yes
Raw Data File Type(s)	raw
Analysis Type Detail	GC-MS
Release Date	2021-07-05
Release Version	1

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	treatment
SA171286	AW_M_CO_6h_054	Control
SA171287	AW_M_CO_6h_053	Control
SA171288	PA_F_CO_2h_099	Control
SA171289	AW_M_CO_6h_052	Control
SA171290	PA_F_CO_24h_097	Control
SA171291	PA_F_CO_24h_095	Control
SA171292	PA_F_CO_24h_096	Control
SA171293	AW_M_CO_6h_051	Control
SA171294	PA_F_CO_24h_098	Control
SA171295	AW_M_CO_6h_050	Control
SA171296	AW_M_CO_2h_044	Control
SA171297	AW_M_CO_2h_043	Control
SA171298	PA_M_CO_24h_132	Control
SA171299	AW_M_CO_2h_045	Control
SA171300	AW_M_CO_2h_046	Control
SA171301	AW_M_CO_6h_049	Control
SA171302	AW_M_CO_2h_048	Control
SA171303	AW_M_CO_2h_047	Control
SA171304	PA_F_CO_24h_094	Control
SA171305	AW_F_CO_24h_001	Control
SA171306	PA_M_CO_2h_138	Control
SA171307	PA_M_CO_2h_139	Control
SA171308	PA_M_CO_2h_140	Control
SA171309	PA_M_CO_2h_137	Control
SA171310	PA_M_CO_2h_136	Control
SA171311	PA_M_CO_24h_133	Control
SA171312	PA_M_CO_24h_134	Control
SA171313	PA_M_CO_2h_135	Control
SA171314	PA_M_CO_6h_141	Control
SA171315	PA_M_CO_6h_142	Control
SA171316	PA_M_CO_24h_130	Control
SA171317	PA_M_CO_24h_129	Control
SA171318	AW_M_CO_24h_041	Control
SA171319	PA_M_CO_24h_131	Control
SA171320	PA_M_CO_6h_146	Control
SA171321	PA_M_CO_6h_143	Control
SA171322	PA_M_CO_6h_144	Control
SA171323	PA_M_CO_6h_145	Control
SA171324	PA_F_CO_24h_093	Control
SA171325	AW_M_CO_24h_042	Control
SA171326	AW_F_CO_2h_007	Control
SA171327	AW_F_CO_2h_008	Control
SA171328	AW_F_CO_2h_009	Control
SA171329	AW_M_CO_24h_040	Control
SA171330	AW_F_CO_24h_006	Control
SA171331	AW_F_CO_24h_004	Control
SA171332	AW_F_CO_24h_005	Control
SA171333	AW_F_CO_2h_010	Control
SA171334	AW_F_CO_6h_018	Control
SA171335	AW_F_CO_6h_013	Control
SA171336	AW_F_CO_2h_012	Control
SA171337	AW_F_CO_6h_014	Control
SA171338	AW_F_CO_6h_015	Control
SA171339	AW_F_CO_6h_017	Control
SA171340	AW_F_CO_6h_016	Control
SA171341	AW_F_CO_24h_003	Control
SA171342	AW_F_CO_24h_002	Control
SA171343	PA_F_CO_2h_101	Control
SA171344	PA_F_CO_2h_102	Control
SA171345	PA_F_CO_2h_100	Control
SA171346	AW_M_CO_24h_037	Control
SA171347	AW_M_CO_24h_039	Control
SA171348	AW_M_CO_24h_038	Control
SA171349	PA_F_CO_2h_103	Control
SA171350	PA_F_CO_2h_104	Control
SA171351	PA_F_CO_6h_109	Control
SA171352	PA_F_CO_6h_110	Control
SA171353	PA_F_CO_6h_108	Control
SA171354	PA_F_CO_6h_107	Control
SA171355	PA_F_CO_6h_105	Control
SA171356	PA_F_CO_6h_106	Control
SA171357	AW_F_CO_2h_011	Control
SA171358	PA_F_NA_6h_125	Naphthalene
SA171359	PA_F_NA_2h_122	Naphthalene
SA171360	PA_F_NA_6h_123	Naphthalene
SA171361	PA_F_NA_6h_124	Naphthalene
SA171362	PA_F_NA_2h_121	Naphthalene
SA171363	PA_F_NA_2h_120	Naphthalene
SA171364	PA_F_NA_6h_127	Naphthalene
SA171365	PA_F_NA_2h_119	Naphthalene
SA171366	PA_F_NA_6h_126	Naphthalene
SA171367	PA_F_NA_6h_128	Naphthalene
SA171368	PA_M_NA_2h_158	Naphthalene
SA171369	pool_168	Naphthalene
SA171370	pool_169	Naphthalene
SA171371	pool_170	Naphthalene
SA171372	pool_167	Naphthalene
SA171373	pool_166	Naphthalene
SA171374	PA_M_NA_6h_164	Naphthalene
SA171375	pool_165	Naphthalene
SA171376	pool_171	Naphthalene
SA171377	pool_172	Naphthalene
SA171378	pool_177	Naphthalene
SA171379	pool_178	Naphthalene
SA171380	pool_176	Naphthalene
SA171381	pool_175	Naphthalene
SA171382	pool_173	Naphthalene
SA171383	pool_174	Naphthalene
SA171384	PA_M_NA_6h_163	Naphthalene
SA171385	PA_M_NA_6h_162	Naphthalene