Summary of Study ST002095
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001328. The data can be accessed directly via it's Project DOI: 10.21228/M82978 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002095 |
| Study Title | Addressing batch effects in large-scale metabolomics with augmented experimental design and meta-analysis (Part 1) |
| Study Summary | Non-polar and polar sequential extracts were collected on three groups of C. elegans: natural isolates, central metabolism mutants, and UDP-glucuronosyltrasnferase mutants. Untargeted LC-MS (HILIC/RP - positive and negative mode) and NMR (CDCl3/H2O) studies were conducted using a Vanquish UHPLC coupled to an Orbitrap Elite MS and NEO 800 MHz Bruker NMR spectrometer, respectively. An augmented design, rank transformation of the raw data, strict feature filtering and QA/QC followed by a meta-analysis was performed on all datasets to demonstrate the benefits of this analysis approach and identify stable, significant features to prioritize for downstream compound identification efforts. |
| Institute | University of Georgia - Complex Carbohydrate Research Center |
| Laboratory | Edison Lab |
| Last Name | Garcia |
| First Name | Brianna |
| Address | 315 Riverbend Road, Athens, GA, 30602, USA |
| brianna.garcia@uga.edu | |
| Phone | 7065424401 |
| Submit Date | 2022-02-22 |
| Num Groups | 3 |
| Total Subjects | 116 |
| Study Comments | Three study groups of C. elegans strains were used: central metabolism mutants (CMM), UDP-glucuronosyltransferase (UGT) mutants, and natural isolates. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2022-04-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Invertebrate; Subject species: Caenorhabditis elegans (Factor headings shown in green)
| mb_sample_id | local_sample_id | set | batch | genotype |
|---|---|---|---|---|
| SA200948 | aos142_ga_ms2_7 | 1 | 1 | CB4856 |
| SA200949 | aos145_ga_ms2_8 | 1 | 1 | CB4856 |
| SA200950 | aos127_ga_ms2_5 | 1 | 1 | CX11314 |
| SA200951 | aos76_ga_ms2_21 | 1 | 1 | CX11314 |
| SA200952 | aos25_ga_ms2_10 | 1 | 1 | CX11314 |
| SA200953 | aos46_ga_ms2_14 | 1 | 1 | DL238 |
| SA200954 | aos65_ga_ms2_19 | 1 | 1 | DL238 |
| SA200955 | aos50_ga_ms2_16 | 1 | 1 | DL238 |
| SA200956 | aos67_ga_ms2_20 | 1 | 1 | N2 |
| SA200957 | aos48_ga_ms2_15 | 1 | 1 | N2 |
| SA200958 | aos58_ga_ms2_18 | 1 | 1 | N2 |
| SA200959 | aos43_ga_ms4_13 | 1 | 1 | PD1074 |
| SA200960 | aos27_ga_ms3_11 | 1 | 1 | PD1074 |
| SA200961 | aos129_ga_ms2_6 | 1 | 1 | PD1074 |
| SA200962 | aos149_ga_ms2_9 | 1 | 1 | PD1074 |
| SA200963 | aos78_ga_ms4_22 | 1 | 1 | PD1074 |
| SA200964 | aos32_ga_ms2_12 | 1 | 1 | RB2011 |
| SA200965 | aos122_ga_ms2_4 | 1 | 1 | RB2011 |
| SA200966 | solera_3 | 1 | 1 | solera |
| SA200967 | aos170_ga_ms2_32 | 1 | 2 | CB4856 |
| SA200968 | aos115_gt_ms1_29 | 1 | 2 | CB4856 |
| SA200969 | aos199_ga_ms2_38 | 1 | 2 | CX11314 |
| SA200970 | aos175_ga_ms2_35 | 1 | 2 | CX11314 |
| SA200971 | aos127_ga_ms3_24 | 1 | 2 | CX11314 |
| SA200972 | aos165_ga_ms2_30 | 1 | 2 | DL238 |
| SA200973 | aos192_ga_ms2_37 | 1 | 2 | DL238 |
| SA200974 | aos174_ga_ms2_34 | 1 | 2 | DL238 |
| SA200975 | aos106_co_ms2_25 | 1 | 2 | N2 |
| SA200976 | aos56_ga_ms2_41 | 1 | 2 | N2 |
| SA200977 | aos113_ga_ms1_28 | 1 | 2 | PD1074 |
| SA200978 | aos188_ga_ms3_36 | 1 | 2 | PD1074 |
| SA200979 | aos99_ga_ms2_26 | 1 | 2 | PD1074 |
| SA200980 | aos53_ga_ms2_40 | 1 | 2 | PD1074 |
| SA200981 | aos200_ga_ms2_39 | 1 | 2 | PD1074 |
| SA200982 | aos167_ga_ms3_31 | 1 | 2 | PD1074 |
| SA200985 | pooled_natural_isolate_42 | 1 | 2 | pooled_natural_isolate |
| SA200986 | pooled_pd1074_43 | 1 | 2 | pooled_pd1074 |
| SA200983 | aos173_ga_ms2_33 | 1 | 2 | RB2011 |
| SA200984 | aos108_ga_ms2_27 | 1 | 2 | RB2011 |
| SA200987 | solera_44 | 1 | 2 | solera |
| SA200988 | aos86_ga_ms2_65 | 2 | 3 | KJ550 |
| SA200989 | aos28_ga_ms2_53 | 2 | 3 | KJ550 |
| SA200990 | aos69_ga_ms1_64 | 2 | 3 | PD1074 |
| SA200991 | aos43_ga_bku_60 | 2 | 3 | PD1074 |
| SA200992 | aos90_ga_ms3_66 | 2 | 3 | PD1074 |
| SA200993 | aos27_ga_ms4_52 | 2 | 3 | PD1074 |
| SA200994 | aos99_ga_ms2_68 | 2 | 3 | PD1074 |
| SA200995 | aos38_ga_ms2_57 | 2 | 3 | PD1074 |
| SA200996 | aos185_ga_ms2_51 | 2 | 3 | PD1074 |
| SA200997 | aos37_ga_ms2_56 | 2 | 3 | RB2347 |
| SA200998 | aos74_ga_ms2_63 | 2 | 3 | RB2347 |
| SA200999 | aos36_ga_ms2_55 | 2 | 3 | RB2347 |
| SA201000 | aos35_ga_ms2_54 | 2 | 3 | RB2550 |
| SA201001 | aos40_ga_ms2_58 | 2 | 3 | RB2550 |
| SA201002 | aos71_ga_ms2_62 | 2 | 3 | RB2550 |
| SA201006 | solera_49 | 2 | 3 | solera |
| SA201003 | aos98_ga_ms2_67 | 2 | 3 | VC1265 |
| SA201004 | aos42_ga_ms2_59 | 2 | 3 | VC1265 |
| SA201005 | aos184_ga_ms2_50 | 2 | 3 | VC1265 |
| SA201007 | aos109_ga_ms2_81 | 2 | 4 | KJ550 |
| SA201008 | aos100_ga_ms2_73 | 2 | 4 | KJ550 |
| SA201009 | aos134_ga_ms2_86 | 2 | 4 | KJ550 |
| SA201010 | aos140_ga_ms3_87 | 2 | 4 | PD1074 |
| SA201011 | aos113_ga_ms1_84 | 2 | 4 | PD1074 |
| SA201012 | aos180_ga_ms2_89 | 2 | 4 | PD1074 |
| SA201013 | aos92_ga_ms2_77 | 2 | 4 | PD1074 |
| SA201014 | aos87_co_ms3_72 | 2 | 4 | PD1074 |
| SA201015 | aos107_ga_bku_80 | 2 | 4 | PD1074 |
| SA201027 | pooled_mutant_90 | 2 | 4 | pooled_mutant |
| SA201028 | pooled_pd1074_91 | 2 | 4 | pooled_pd1074 |
| SA201016 | aos95_ga_ms2_79 | 2 | 4 | RB2347 |
| SA201017 | aos116_ga_ms2_83 | 2 | 4 | RB2347 |
| SA201018 | aos171_ga_ms2_88 | 2 | 4 | RB2347 |
| SA201019 | aos82_ga_ms2_75 | 2 | 4 | RB2347 |
| SA201020 | aos81_ga_ms2_71 | 2 | 4 | RB2550 |
| SA201021 | aos111_ga_ms2_82 | 2 | 4 | RB2550 |
| SA201022 | aos96_ga_ms2_74 | 2 | 4 | RB2550 |
| SA201029 | solera_92 | 2 | 4 | solera |
| SA201023 | aos84_ga_ms2_76 | 2 | 4 | VC1265 |
| SA201024 | aos73_ga_ms2_70 | 2 | 4 | VC1265 |
| SA201025 | aos125_ga_ms2_85 | 2 | 4 | VC1265 |
| SA201026 | aos93_ga_ms2_78 | 2 | 4 | VC1265 |
| SA201030 | aos79_ga_ms2_116 | 3 | 5 | AUM2073 |
| SA201031 | aos51_ga_ms2_109 | 3 | 5 | AUM2073 |
| SA201032 | aos91_ga_ms2_119 | 3 | 5 | AUM2073 |
| SA201033 | aos75_ga_ms4_115 | 3 | 5 | PD1074 |
| SA201034 | aos41_ga_ms2_104 | 3 | 5 | PD1074 |
| SA201035 | aos44_ga_ms2_106 | 3 | 5 | PD1074 |
| SA201036 | aos69_ga_ms3_114 | 3 | 5 | PD1074 |
| SA201037 | aos87_ga_ms3_118 | 3 | 5 | PD1074 |
| SA201038 | aos196_ga_ms2_101 | 3 | 5 | PD1074 |
| SA201039 | aos181_ga_ms2_99 | 3 | 5 | PD1074 |
| SA201040 | aos83_ga_ms2_117 | 3 | 5 | RB2055 |
| SA201041 | aos26_ga_ms2_102 | 3 | 5 | RB2055 |
| SA201042 | aos45_ga_ms2_110 | 3 | 5 | RB2055 |
| SA201051 | solera_97 | 3 | 5 | solera |
| SA201043 | aos39_ga_ms2_103 | 3 | 5 | UGT49 |
| SA201044 | aos49_ga_ms2_108 | 3 | 5 | UGT49 |
| SA201045 | aos66_ga_ms2_112 | 3 | 5 | UGT60 |
| SA201046 | aos33_ga_ms2_105 | 3 | 5 | UGT60 |
